Check QC Product
Perform the following to check the quantity and quality of the QC product.
The QC product is stable at -25°C to -15°C for up to four weeks. If necessary, you can submit the QC product with the final libraries when using a core lab for fragment analysis.
| 1. | Quantify 2 μl of each sample using a Qubit High Sensitivity kit. |
| 2. | Assess average fragment size using an Agilent Bioanalyzer or Tapestation. |
| • | If necessary, dilute samples to make sure that they are within the appropriate range for the device. |
| • | Load an appropriate volume of the purified library on the fragment analyzer. |
Recommended loading concentration is 1–10 ng/µl on Tapestation or 1–2 ng/µl on Bioanalyzer.
Loading less than 1 ng/µl may not give accurate results.
| 3. | Proceed to library preparation upon successful cDNA QC. The average fragment size for cDNA should be > 500 bp as determined by the fragment analyzer region table (set for 200–5000 bp). Average size may vary depending on cell type and experimental condition. |
Representative cDNA traces for a
