Prepare for Protocol

This preparation is required to perform the protocol steps leading up to the next safe stopping point.

Remove sample from storage.

Remove third-party reagents from storage.

Third-Party Reagents
Reagent	Storage	Thaw Instructions
RNase Inhibitor*	Varies for third‑party reagents	Keep on ice. Refer to the manufacturer instructions as needed.
[Cryopreserved cells] Thawing media	Varies for third‑party reagents	Refer to the manufacturer instructions as needed.

* For cell and nuclei suspensions, use user-supplied RNase Inhibitor.

Remove the reagents from the box and thaw as follows.

15°C to 30°C Storage
Reagent	Box Name	Thaw Instructions
PAR	Illumina Single Cell 3′ RNA Capture, T100, Box 1	Use at room temperature.
CLB3	Illumina Single Cell 3′ RNA Capture, T100, Box 1	Use at room temperature.

-25°C to -15°C Storage
Reagent	Box Name	Thaw Instructions
[Cell preparation] CSB	Illumina Single Cell 3′ RNA Capture, T100, Box 3	For cryopreserved cells—Warm to 37°C in a water bath. For fresh cells—Keep cold.
[Nuclei preparation] NSB	Illumina Single Cell 3′ RNA Capture, T100, Box 3	Use at room temperature.
RNI*	Illumina Single Cell 3′ RNA Capture, T100, Box 3	Keep on ice.
25% BSA	Illumina Single Cell 3′ RNA Capture, T100, Box 3	Thaw on ice.

* For capture and lysis, use Illumina-supplied RNI.

-85°C to -65°C Storage
Reagent	Box Name	Thaw Instructions
T100 PIP	Illumina Single Cell 3′ RNA Capture, T100, Box 4	Thaw one tube per sample on ice. Stable on ice for up to 5 hours.