Dry Bath Operation
The protocol requires a dry bath with 0.5 ml tube block. Make sure that the appropriate block is installed.
Dry Bath Control
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Lid temperature is set manually and is independent from the dry bath programs. Understand how to configure lid mode and temperature before starting the protocol. |
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The dry bath interface displays Lid On when the lid is off and Lid Off when the lid is on. |
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For nuclei lysis and reverse transcription, use the specific temperature control mode to set the lid temperature to 105°C. When set to this mode, the lid temperature box shows the set value (for example, 105.0). |
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For cell lysis, use the offset temperature control mode to set the lid temperature to 5°C higher than the heat block. When set to this mode, the lid temperature box shows the temperature offset (for example +5.0). |
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To change the lid mode, select Edit, and then select LidMode to toggle between 105°C and +5.0, and then select Save/Return. |
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The lid temperature only climbs to 105°C during incubation temperatures of 35°C or higher. |
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The dry bath is preprogrammed with nuclei lysis and cell lysis incubation protocols. Do not modify the protocols. |
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Preheat and hold settings are depicted as 00:00. |
Dry Bath Programs
