User-Supplied Consumables & Equipment

0.2 ml PCR 8-tube strips and caps, emulsion safe

1.5 ml Safe-Lock PCR Clean tubes, emulsion safe

[Fresh cells] 5 ml polypropylene centrifuge tubes, sterile

[Cryopreserved cells] 15 ml polypropylene centrifuge tubes, sterile

Absolute ethanol, molecular biology grade

[Isolated nuclei input] BSA, molecular biology grade

Cell counting materials (eg, AO/PI)

Nuclease-free water

Nuclease-free 1X low EDTA TE Buffer (IDTE), pH 8.0, 10 mM Tris, 0.1 mM EDTA

Pipette tips, filtered, low retention, sterilized, 20 μl

Pipette tips, filtered, low retention, sterilized, 200 μl

Pipette tips, filtered, low retention, sterilized, 1000 μl

Pipette tips, wide bore, filtered, low retention, sterilized, 200 μl

Pipette tips, wide bore, filtered, low retention, sterilized, 1000 μl

PCR tube strips and caps, or PCR reaction plates (if you have a compatible magnetic bead rack)

Quantification consumables, depending on system:

Qubit 1X dsDNA High Sensitivity Assay Kit

Qubit Assay Tubes

[Isolated nuclei input] RNase Inhibitor

0.2 ml/1.5 ml Magnetic Separation Combo Rack

Benchtop centrifuge with swinging bucket rotor, suitable for 5 ml, 15 ml tubes

Benchtop mini microcentrifuge 2000 × g, suitable for 1.5 ml tubes, 0.5 ml tubes and PCR tube strips.

Hemocytometer or automated cell counter

Ice bucket or cold blocks, suitable for 0.2 ml and 0.5 ml PCR tubes and 1.5 ml microcentrifuge tubes

Laboratory balance

Micropipettes, 1–1000 µl capabilities

Quantification system, either of the following: 

Qubit Fluorometer

Thermal cycler