Prepare for Library Prep Protocol

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The following steps are performed in the pre-amplification area.

1. Remove the cfDNA from storage and thaw on ice.
2. Remove the reagents from the box and prepare as follows.
Room Temperature Storage

Reagent

Box Name

Instructions

Protocol Step

IPB

Box 2

Use at room temperature.

Clean Up Ligation
-25°C to -15°C Storage

Reagent

Box Name

Instructions

Protocol Step

ATL4

Box 1

Thaw at room temperature.

Perform A-Tailing

EPM4

Box 1

Thaw on ice.

Index PCR

ERP6

Box 1

Thaw on ice.

Prepare cfDNA and Perform End Repair

LIGX

Box 1

Thaw on ice.

Ligate Adapters

RSB

Box 1

Bring to room temperature.

Prepare cfDNA and Perform End Repair, Index PCR

UD Index Plate

N/A

Thaw at room temperature.

Index PCR

UMI DNA

N/A

Thaw at room temperature.

Ligate Adapters