Product Contents

Completing the TruSight Oncology 500 ctDNA v2 protocol requires library prep and enrichment reagents, UMI DNA index anchors (UMI DIA), and index adapters.

Component

Kit Options

Catalog #

Library prep and enrichment reagents

TruSight Oncology 500 ctDNA v2 Kit (24 Samples)

20105899

TruSight Oncology 500 ctDNA v2 Automation Kit (48 Samples)

20105900

Index Adapters

IDT for Illumina UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20034701

IDT for Illumina UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20034702

IDT for Illumina UMI DNA/RNA UD Indexes Set A for Automation (96 Indexes, 96 Samples)

20066404

IDT for Illumina UMI DNA/RNA UD Indexes Set B for Automation (96 Indexes, 96 Samples)

20063213
Box 1—TruSight Oncology 500 ctDNA v2 Kit, Store at -25°C to -15°C

Tube Quantity

Reagent

Description

24 Samples

48 Samples

1

3

ATL4

A-Tailing Mix 4

1

2

EPM4

Enhanced PCR Mix

1

3

ERP6

End Repair Mix 6

1

2

LIGX

DNA Ligase X

1

1

RSB

Resuspension Buffer

Box 2—TruSight Oncology 500 ctDNA v2 Kit, Store at Room Temperature

Tube Quantity

Reagent

Description

24 Samples

48 Samples

1

3

IPB

Illumina Purification Beads

Box 3—TruSight Oncology 500 ctDNA v2 Kit, Store at -25°C to -15°C

Tube Quantity

Reagent

Description

24 Samples

48 Samples

1

3

EE1

Enrichment Elution Buffer 1

1

1

EEW

Enhanced Enrichment Wash

1

2

EPM4

Enhanced PCR Mix

1

2

HP3

2 N NaOH

1

1

LNA1

Library Normalization Additives

1

3

NHB2

Hyb Buffer 2 + IDT NXT Blockers

1

6*

OPD2

Enrichment Probe Panel

2

4

PPC3

PCR Primer Cocktail 3

1

2

RSB

Resuspension Buffer

*OPD2 is shipped in a separate reagent box for automation kit (48 samples).

Box 4—TruSight Oncology 500 ctDNA v2 Kit

Tube Quantity

Reagent

Description

Storage Temperature

24 Samples

48 Samples

1

2

EHB2

Enrich Hyb Buffer 2

2°C to 8°C

1

2

IPB

Illumina Purification Beads

Room temperature

1

1

LNB1

Library Normalization Beads

2°C to 8°C

1

1

LNS1

Library Normalization Storage Buffer 1

2°C to 8°C

2

4

LNW1

Library Normalization Wash 1

2°C to 8°C

2

5

SMB3

Streptavidin Magnetic Beads

2°C to 8°C

Store at -25°C to -15°C. Each kit contains one plate of IDT for Illumina UMI DNA Index Anchors and one plate of IDT for Illumina DNA/RNA UD Indexes Set A or B.

Description

IDT for Illumina UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples) (UDP0001–UDP0096)

IDT for Illumina UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples) (UDP0097–UDP0192)

IDT for Illumina UMI DNA/RNA UD Indexes Set A, Automation (96 Indexes, 96 Samples) (UDP0001–UDP0096)

IDT for Illumina UMI DNA/RNA UD Indexes Set B, Automation (96 Indexes, 96 Samples) (UDP0097–UDP0192)