Prepare for Protocol
This preparation is required to perform the protocol steps leading up to the next stopping point.
If using samples known to contain only DNA, skip to Tagment DNA.
| 1. | Remove reagents from storage. |
| 2. | Remove the reagents from the box and prepare as follows. |
|
Reagent |
Box Name |
Instructions |
|---|---|---|
|
IPB |
Illumina Purification Beads |
Use at room temperature. |
|
Reagent |
Box Name |
Instructions |
|---|---|---|
|
EPH3 |
Illumina cDNA Synthesis |
Thaw at room temperature. |
|
FSA |
Illumina cDNA Synthesis |
Thaw on ice. |
|
RSB |
Illumina cDNA Synthesis |
Thaw at room temperature. |
|
RVT |
Illumina cDNA Synthesis |
Thaw on ice. |
|
SMM |
Illumina cDNA Synthesis |
Thaw on ice. |
