Protocol
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This section describes the lllumina Viral Surveillance Panel v2 kit protocol and provides instructions for preparing and enriching libraries.
| • | Review the complete sequencing workflow, from sample through analysis, to ensure compatibility of products and experiment parameters. |
Pooling Preparation
It is recommended to pool samples with similar viral titer for 3-plex enrichment. Pooling samples with above average and below average viral titer might bias results. Single-plex enrichment is recommended for samples with very low viral titer.
When pooling libraries, record information about your samples before starting library prep. Use a recording tool compatible with your sequencing system and libraries. For compatibility information, refer to the Illumina Viral Surveillance Panel v2 support pages or the support pages for your system.
The protocol uses Illumina DNA/RNA UD Indexes to index libraries. These
| • | For strategies on forming low-plex, color-balanced pools, refer to the Index Adapters Pooling Guide. |
| • | For index adapter sequences and how to record them, refer to Illumina Adapter Sequences. |
lllumina Viral Surveillance Panel v2 kit Workflow
The following diagram provides an overview of the VSP v2 kit protocol using a single sample. Safe stopping points are marked between steps.
