Facility Requirements

Equipment	Height	Width	Depth	Weight
VeriSeq NIPT Onsite Server v2 – RUO	43.8 cm (17.3 in)	17.8 cm (7 in)	63.5 cm (25 in)	25.9 kg (57 lb)
VeriSeq NIPT Microlab STAR with autoload	90.3 cm (35.6 in)	199 cm (78.3 in)	100.6 cm (39.6 in)	160 kg (353 lb)

VeriSeq NIPT Onsite Server v2 – RUO Placement Requirements

Position the VeriSeq NIPT Microlab STAR to allow for the following requirements:

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Power cord connection to two power outlets and quick disconnection.

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Proper ventilation.

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Two standard power sockets within 1.8 m (6 ft) of the server.

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One-network socket located within 1.8 m (6 ft) of the server or longer if using a user-supplied network cable.

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One static-reserved IP address.

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A rack unit size of 4U, if you position the server in a rack.

If you want to order server rails, notify your Illumina Support Personnel.

A server positioned upright must be accessible from all sides with the following minimum clearance dimensions:

Access	Minimum Clearance
Sides	61.0 cm (24.0 in) on each side of the server
Rear	10.2 cm (4.0 in) behind the server
Top	61.0 cm (24.0 in) above the server

VeriSeq NIPT Microlab STAR Placement Requirements

Position the VeriSeq NIPT Microlab STAR to allow for the following factors:

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Proper ventilation.

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Five standard power sockets within 1.8 m (6 ft).

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Two additional standard power sockets for service purposes within 1.8 m (6 ft).

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One-network socket located within 1.8 m (6 ft) or longer, if using a user-supplied network cable.

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Bench space to the right or left of the instrument to accommodate the PC and monitor.

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Space underneath the instrument to accommodate the vacuum pump, waste bins, two waste bottles, and Inheco Multi TEC Control Unit (accessory equipment provided with purchase of the VeriSeq NIPT Microlab STAR).

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Clearance for a waste bin underneath the CO-RE head tips waste chute to the left of the instrument (~26 cm or 10.2 in).

Accessory Equipment	Height	Width	Depth
Inheco Multi TEC Control Unit	26.4 cm (10.4 in)	18.5 cm (7.3 in)	24.9 cm (9.8 in)
Vacuum pump	25 cm (9.8 in)	22 cm (8.7 in)	23 cm (9.1 in)
Waste bottle	41 cm (16.1 in)	18 cm (7.1 in)	18 cm (7.1 in)

Reagent Storage Requirements

The following tables provide storage temperature and dimensions for the VeriSeq NIPT Solution v2 – RUO reagents. Make sure to include storage requirements for your sequencing instrument reagent kit.

TG VeriSeq NIPT Sample Prep Kit (24), Part # 20031755
Part #	Description	Dimensions	Storage
20031687	TG VeriSeq NIPT Extraction Box (24)	16 cm × 15 cm × 11 cm (6.3 in × 5.9 in × 4.3 in)	Room temperature
20031688	TG VeriSeq NIPT Library Prep Box (24)	16 cm × 15 cm × 11 cm (6.3 in × 5.9 in × 4.3 in)	-25°C to ‑15°C
15068656	TG VeriSeq NIPT Accessory Box	16 cm × 12 cm × 14 cm (6.3 in × 4.7 in × 5.5 in)	2°C to 8°C
15071541	TG VeriSeq NIPT Workflow Tubes and Labels	17 cm × 10 cm × 1 cm (6.7 in × 3.9 in × 0.4 in)	Room temperature

TG VeriSeq NIPT Sample Prep Kit (48), Part # 15068653
Part #	Description	Dimensions	Storage
15068658	TG VeriSeq NIPT Extraction Box (48)	16 cm × 15 cm × 11 cm (6.3 in × 5.9 in × 4.3 in)	Room temperature
15068659	TG VeriSeq NIPT Library Prep Box (48)	16 cm × 15 cm × 11 cm (6.3 in × 5.9 in × 4.3 in)	-25°C to ‑15°C
15068656	TG VeriSeq NIPT Accessory Box	16 cm × 12 cm × 14 cm (6.3 in × 4.7 in × 5.5 in)	2°C to 8°C
15071541	TG VeriSeq NIPT Workflow Tubes and Labels	17 cm × 10 cm × 1 cm (6.7 in × 3.9 in × 0.4 in)	Room temperature

TG VeriSeq NIPT Sample Prep Kit (96), Part # 15068652
Part #	Description	Dimensions	Storage
15068654	TG VeriSeq NIPT Extraction Box (96)	16 cm × 15 cm × 11 cm (6.3 in × 5.9 in × 4.3 in)	Room temperature
15068655	TG VeriSeq NIPT Library Prep Box (96)	16 cm × 15 cm × 11 cm (6.3 in × 5.9 in × 4.3 in)	-25°C to ‑15°C
15068656	TG VeriSeq NIPT Accessory Box	16 cm × 12 cm × 14 cm (6.3 in × 4.7 in × 5.5 in)	2°C to 8°C
15071541	TG VeriSeq NIPT Workflow Tubes and Labels	17 cm × 10 cm × 1 cm (6.7 in × 3.9 in × 0.4 in)	Room temperature

Pre-PCR Area

Before beginning work in the lab, establish dedicated areas and lab procedures to prevent PCR product contamination. PCR products can contaminate reagents, instruments, and samples. Cross-contamination can delay normal operations and cause inaccurate results.

Use the following guidelines to avoid cross-contamination.

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Establish a pre-PCR area with dedicated entrances for pre-PCR processes.

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Make sure that lab personnel do not need to pass through any post-PCR lab areas to access the pre-PCR area.

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Place the VeriSeq NIPT Microlab STAR in the pre-PCR area.

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Do not pass material or equipment from any post-PCR area to the pre-PCR area.

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Because the VeriSeq NIPT Workflow Manager workflow does not include a PCR step, the next-generation sequencing system can be located in the pre-PCR area, unless it is being used for other applications.

Example Lab Layout

The following figure provides an example layout for one VeriSeq NIPT Microlab STAR, two NextSeq 500/550 instruments, and ancillary lab equipment. This example layout requires approximately 35 square meters (377 sq ft). The VeriSeq NIPT Onsite Server v2 – RUO and UPS do not need to be placed in the lab and are intentionally not shown in the example layout.

VeriSeq NIPT Solution v2 – RUO Example Lab Layout (not to scale)

Barcode Printing Requirements

Use the following guidelines when printing barcode labels for the Streck blood tube.

Barcode Specifications
Specification	Description
Type	Black bars with white background.
Symbology	Code 128, Subset B. This symbology covers ASCII characters 32 to 127 (0–9, A–Z, a–z) and special characters. For specific bar code requirements for the VeriSeq NIPT Workflow Manager software and Analysis Control Software, refer to the sample_barcode description in the Sample Sheet Input section.
Code density, tolerance	Minimum module width (x dimension) including a print tolerance: ≥ 0.1651 mm (0.0065 in). Maximum module width (x dimension) including a print tolerance: ≤ 0.508 mm (0.02 in). Best reading performance with x dimension ≥ 0.254 mm (0.01 in).
Number of check characters	One character.
Quiet zone	≥ 10 times the x dimension, but at least 3 mm (0.11811 in).
Print quality	The barcode print must be of a high quality. A printed barcode with an ANSI/CEN/ISO grade A or B is required. Offset, typographic, intaglio, and flexographic printing are suitable. Mechanical dot matrix and thermo matrix printing are not suitable. The surface can be treated, sealed, or plastic-coated.

Barcode Dimensions