VeriSeq NIPT Microlab STAR Troubleshooting

Process Step

Error Code

Error Dialog

Description

User Resolution

Batch Creation

EM0044

The Batch ID entered contains forbidden characters.

The VeriSeq NIPT Solution v2 – RUO only accepts numbers, letters, underscores, and dashes for all data fields.

Rename batch using a name that does not contain special characters.

Batch Creation

EM0051

The Batch ID is greater than 26 characters in length.

The VeriSeq NIPT Solution v2 – RUO limits the length of batch names to 26 characters or less.

Rename batch using a name that is fewer than 26 characters.

Batch Creation

EM0076

Unable to connect to VeriSeq NIPT Onsite Server v2 – RUO

The VeriSeq NIPT Onsite Server v2 – RUO is not responding to data requests from the Workflow Manager.

1. Make sure that the ML STAR is connected to the network.

2. Make sure that the VeriSeq NIPT Onsite Server v2 – RUO is on.

3. Check that the ML STAR can connect to the VeriSeq NIPT Onsite Server v2 – RUO (via ping request).

4. If previous steps do not resolve the issue, contact Illumina Technical Support.

Batch Creation

EM0118

This batch has been failed and cannot be further processed.

The specified batch has already failed and cannot be processed any further.

The batch record on the VeriSeq NIPT Onsite Server v2 – RUO indicates that the selected batch has failed. No further processing is allowed. Create another batch with the required samples.

Batch Creation

Not applicable

This batch has already completed processing. Would you like to repool?

The indicated batch has processed through pooling. The only allowable processing is repool.

Repool as follows.

• Select Re-Pool.
• Abort method and ensure that batch name is correct before repooling.

Plasma Isolation

WP0087

Duplicate sample barcodes loaded.

Samples with identical barcodes have been loaded onto the system.

1. Follow the Workflow Manager prompts to identify which samples are duplicates.

2. Remove the duplicates and relabel or replace them.

3. Reload the samples.

Plasma Isolation

EP0102

Samples specified in the Sample Sheet were not loaded.

Samples included in the sample sheet were not included in the loaded barcodes.

1. Follow the Workflow Manager prompts to identify missing samples.

2. Do one of the following options:

• Add missing samples to the batch and reload samples.
• Abort method, modify the sample sheet as needed. Restart method.

Plate Load

Not applicable

Venus Barcode Mask Error

The Workflow Manager enforces correct plate-to-batch association using Venus barcode masks.

1. Check plate placement to confirm that the plate layout is correct.

2. Make sure that the plate loaded is the correct plate for the indicated batch.

cfDNA Extraction

WE0150

Pressure in the vacuum chamber is too low.

The Workflow Manager does not proceed if the resting vacuum line pressure sense is < 400 Torr.

1. Check for kinks or other obstructions in the vacuum line.

2. Open the waste line release clips, allow for pressure to release, and then fully close the release line clips.

3. Make sure that the vacuum controller and pump are turned on.

4. Check the vacuum waste bottle. If the waste bottle is more than half full, empty the waste bottle.

5. If the problem persists, contact Illumina Technical Support.

cfDNA Extraction

WE0153

Pressure in the vacuum chamber is too high.

If the measured vacuum pressure is too high before the pressure control starts, the system might be malfunctioning.

On the back of the controller, make sure that all vacuum connections and lines are secure.

cfDNA Extraction

WE0996

Vacuum failed to seal.

The seal failure must be resolved before continuing.

Verify that seal failure is resolved before selecting OK.

1. Make sure thatVeriSeq NIPT Microlab STAR instrument is in a safe state to allow user intervention.

2. Make sure that the binding plate is flush against the vacuum manifold. With a gloved hand, forcibly press down on the binding plate.

3. Listen for the vacuum hum and observe the flow of water through the binding plate.

4. Open the trace view on the Workflow Manager. After the actual pressure reading reaches at least 50 pressure units less than the ambient reading, select OK to continue with cfDNA Extraction.

5. If the required pressure reading is not reached during the allotted time, select OK to continue with the first lysate load.

6. Pause the method after the lysate is dispensed onto the binding plate. Reseat and forcibly press down on the binding plate.

7. If the lysate fails to flow through the plate, contact Illumina Technical Support.

cfDNA Extraction

WM0219

If Vacuum is on, manually rest the pump.

The vacuum might stay on after a method abort during extraction.

1. On the Vacuum Controller, press the Power button to shut off the vacuum.

2. Wait 10 seconds, and then press the Power button again to turn on the vacuum.

cfDNA Extraction

EE0477

An error has occurred while moving a plate.

(iSWAP error)

If an iSWAP error is encountered (plate drop, failed to pick up, etc), the system prompts you to complete the plate move manually.

Make sure that the plate can be recovered (no spilled material).

• If the plate cannot be recovered, abort the run.
• If the plate can be recovered, follow the displayed instructions to complete the plate transfer manually.

cfDNA Extraction

EE0519

Scanned barcode does not match Binding Plate barcode on record.

The loaded Binding plate does not match the barcode of the plate removed.

Make sure that the plate being loaded matches the recorded barcode (refer to the trace log for the expected barcode).

API

 

 

 

 

EA0372

Unable to connect to the data server.

The VeriSeq NIPT Onsite Server v2 – RUO is not responding to data requests from the Workflow Manager.

 

1. Make sure that the ML STAR is connected to the network.

2. Make sure that the VeriSeq NIPT Onsite Server v2 – RUO is on.

3. Check that the ML STAR can connect to the VeriSeq NIPT Onsite Server v2 – RUO (via ping request).

EA0774

Connection Error.

The API server connection failed to validate.

The VeriSeq NIPT Onsite Server v2 – RUO has stopped responding to data requests from the Workflow Manager.

Make sure that:

1. Make sure that the ML STAR is connected to the network.

2. Check that the ML STAR can connect to the VeriSeq NIPT Onsite Server v2 – RUO (via ping request).

3. Make sure that the VeriSeq NIPT Onsite Server v2 – RUO is on.

EA0780

403: Invalid Request

The current transaction is not valid.

The data sent violates the system workflow logic.

Consult the error details for more information. Common causes involve inputs that are too long or violate the acceptable character list.