VeriSeq NIPT Onsite Server v2 – RUO

The VeriSeq NIPT Assay Software makes specific folders on the Onsite Server available to the user. These folders can be mapped using a Samba share protocol to any workstation or laptop on the local network.

Folder Name	Description	Access
Input	Contains sequencing data generated by the NextSeq 500/550 mapped to the server.	Read and write.
Output	Contains all software-generated reports.	Read only.
Backup	Contains database backups.	Read only.

The VeriSeq NIPT Assay Software maintains a local database where the library information, sequencing run information, and analysis results are persisted. The database is an integral part of the VeriSeq NIPT Assay Software and is not accessible to the user. The system maintains an automatic mechanism for database backup on the Onsite Server. In addition to the following database processes, users are encouraged to back up the database regularly to an external location.

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Database backup—A snapshot of the database is automatically saved on an hourly, a daily, a weekly, and a monthly basis. Hourly backups are removed after a daily backup is created. Likewise, the daily backups are removed when the weekly backup is ready. The weekly backups are removed after a monthly backup is created, and only one monthly backup is kept. The recommended practice is to create an automated script that can persist the backup folder on a local NAS.

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Database restore—The database can be restored from any given backup snapshot. Restores are done by Illumina Field Service Engineers only.

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Data backup—Although the Onsite Server can be used as the main storage point for sequencing runs, it can only store approximately 300 runs. You can set up an automated data backup that runs on a continuous basis to another long-term storage device or an NAS.

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Maintenance—Other than data backup, the Onsite Server does not require the user to perform any maintenance. Updates for the VeriSeq NIPT Assay Software or the Onsite Server itself are provided by Illumina Technical Support.

Consult your local IT site archiving policy to determine how to archive the input and output directories. The VeriSeq NIPT Assay Software monitors the remaining disk space in the input directory and notifies users by email when the remaining storage capacity goes below 1 TB.

Do not use the Onsite Server for data storage. Transfer data to the Onsite Server and archive on a regular schedule.

A typical sequencing run that is compatible with the cfDNA analysis workflow requires 25–30 GB for NextSeq 500/550 runs. The actual run folder size depends on final cluster density.

Only archive data when the system is idle and no analysis or sequencing runs are in progress.

The Onsite Server has three folders that can be individually mapped to any computer with Microsoft Windows:

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input—Maps to the sequencing data folders. Mount on the computer connected to the sequencing system. Configure the sequencing system to stream data to the input folder.

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output—Maps to the server analysis reports and assay process reports.

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backup—Maps to the database backup files.

Map each folder as follows.

1.

Log in to the computer within the Onsite Server subnetwork.

2.

Right-click Computer, and select Map network drive.

3.

Select a letter from the Drive drop-down list.

4.

In the Folder field, enter \\<VeriSeq NIPT Onsite Server v2 – RUO IP address>\<folder name>.

For example: \\10.50.132.92\input.

5.

Enter the user name and password (as an active administrator) of the VeriSeq NIPT Assay Software v2. Successfully mapped folders appear mounted on the computer. If the role, active status, or the password of the administrator changes, the active connection of the mapped server is terminated.

Successfully mapped folders appear mounted on the computer.

To reboot the server:

1.

On the Settings drop-down list, select Reboot Server.

2.

Select Reboot to reboot the system, or Cancel to exit without rebooting.

3.

Enter a reason for shutting down the server.

The reason is logged for troubleshooting purposes.

In the event of a power outage or accidental shutdown by the user during an analysis run, the system does as follows:

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Automatically restarts the VeriSeq NIPT Assay Software upon reboot.

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Recognizes that the analysis run failed and resubmits the run to the queue for processing.

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Generates output when analysis successfully completes.