Sample, Batch, and Pool Invalidation

Depending on the sample processing step, you can invalidate an individual sample, a batch, or a sample pool. After invalidation, the sample, batch, or pool is no longer processed.

Before generating data output, you can use either the VeriSeq NIPT Method or Batch Manager to invalidate one or more samples.

Invalidation Using VeriSeq NIPT Method 

To invalidate samples, complete the following steps during sample processing.

1. In the Well Comments window at the end of each Workflow Manager process, select the individual wells to fail, and then select OK.
2. Select at least one annotation from the drop-down menus, or select the Other checkbox and enter a comment.
3. Select the Fail Sample checkbox, and select OK.
4. Confirm that the system is to fail the sample.

Invalidation Using Batch Manager

Use Batch Manager to invalidate the following:

• A sample
• A batch, before the Pool step is complete.
• A sample pool, after the Pool step is complete and before generating the analysis output.

Before running the Batch Manager, exit any currently running methods.

To access Batch Manager, use one of the following actions:

• On the App Launcher, select VeriSeq NIPT Batch Manager.
• On a computer connected to the network, browse to C:\Program Files (x86)\HAMILTON\Methods\VeriSeqNIPT and open the Batch Manager method file (VeriSeqNIPT_Batch_Manager.med) with the Hamilton Run Controller.
1. Access the Batch Manager.
2. Enter the batch ID and user name or operator initials, and select OK.
3. On the batch plate diagram, select the well position associated with the failed sample.
4. Confirm that the correct sample is displayed, and select Invalidate Sample.
5. Enter a reason for failure, and select Invalidate.

On the batch plate diagram, the invalidated sample changes from green to red, and the status label changes from valid to failed.

1. Access the Batch Manager.
2. Enter the batch ID and user name or operator initials, and select OK.
3. On the batch plate diagram, select Invalidate Batch.
4. Enter a reason for failure, and select Invalidate.

On the batch plate diagram, if no valid pools exist for the batch, all samples change from green to red. Valid pools within the batch remain valid.

1. Access the Batch Manager.
2. Enter the batch ID and user name or operator initials, and select Pool Manager.
3. Scan the barcode of the pool.
4. Enter user name or operator initials, and select OK.
5. Enter a reason for failure, and select Invalidate.