Sequencing QC

Sequencing QC metrics identify flow cells that are likely to fail analysis with high probability. The cluster density, percent reads passing filter (PF), prephasing, and phasing metrics describe the general sequencing data quality and are common to many next-generation sequencing applications. The predicted aligned reads metric estimates the flow cell level of the sequencing depth. If low-quality data fails the predicted aligned reads metric, processing the run is terminated. For more information, refer to Sequencing QC Metrics and Boundaries.