Troubleshooting

If a minor error occurs, follow any troubleshooting commands in the error message in the instrument software. Refer to the following table for additional corrective actions to address the issue.

If an error cannot be corrected, contact Illumina Technical Support.

Corrective Actions for Troubleshooting

Problem or Error

Corrective Measures

System liquid leakage

Switch off instrument immediately. Perform decontamination and/or maintenance and contact Illumina Technical Support.

Communication error, power or communication is interrupted, or no communication

Power on the instrument.
Check instrument cable and plug.
Switch off the instrument and PC. Wait until the status lamp is dark and then switch on the instrument and PC.
Make sure that the water circulator is in Standby mode (on) and has not been turned off completely.
Power cycle the water circulator using the large main switch on the back of the circulator.

Initialization error

Make sure that the arms can move freely so that other objects do not obstruct their movement range.
Make sure that the front safety door is securely closed on both sides.
Make sure that the water circulator is in Standby mode (on) and has not been turned off completely.

Front safety panel does not unlock properly

Switch off the instrument. If front safety panel does not unlock, contact Illumina Technical Support.

Front safety panel does not lock properly

Make sure that the front safety door is securely closed on both sides.
Power cycle the instrument and test the front safety panel again during a run to see if it locks correctly.
Switch off the instrument and contact Illumina Technical Support.

Positioning error for PosID3, LiHa, or RoMa (for example, crash, failure to move)

Check for obstacles.
Check container, rack, and carrier positions.
If problem persists, contact Illumina Technical Support.

Barcode not read

Check barcode label position on container. For more information, refer to Barcodes on Containers and Carriers.
Check if barcode type is permissible.
Check container position on carrier.
Check label quality and test a new barcode label.
Check settings in application software.
Check and clean both PosID3 or Keyence scanner windows and make sure their lasers are firing properly.
If barcode still does not read, manually enter the barcode in ILASS.

Carrier or tube presence not detected

Clean the No Tube sensor on the PosID3.
Make sure the PosID3 reflector is in place and carefully clean its reflective surface.
If the problem persists, contact Illumina Technical Support.

Unusual noise during PosID3, RoMa, or LiHa movement

Contact Illumina Technical Support.

Overflow of wash station

Check the wash station outlet tubing for any kinks.
Make sure the waste tube is not below liquid surface in the waste container.
Clean the wash station and check for any obstructions.
Use a waste container with fixed wash tubing inlet.

Damaged or bent tip.

Contact Illumina Technical Support.

ILASS error

Refer to the Illumina Lab Automation Software Solution Product Documentation (document # 200015136).
If problem persists, contact Illumina Technical Support.

Insufficient access rights to run Freedom EVO2

If permission settings have been altered, check and correct those settings.
Contact Illumina Technical Support.

EVOWare warnings regarding folders/files in your virtual store

Check the following path for a folder labeled Tecan and delete it: C:\Users\<user>\AppData\Local\VirtualStore\ProgramData\.
If the error persists, contact Illumina Technical Support.