Sequences for AmpliSeq for Illumina Panels

The AmpliSeq for Illumina CD and UD index adapters are arranged in the plate to enforce the recommended pairing strategy.

The following sequence is used for Read 1 and Read 2 adapter trimming.

CTGTCTCTTATACACATCT

CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG

i7 Index Name

i7 Bases for Sample Sheet

Q7005

GTGAATAT

Q7006

ACAGGCGC

Q7007

CATAGAGT

Q7008

TGCGAGAC

Q7015

TCTCTACT

Q7016

CTCTCGTC

Q7017

CCAAGTCT

Q7018

TTGGACTC

Q7023

GCAGAATT

Q7024

ATGAGGCC

Q7025

ACTAAGAT

Q7026

GTCGGAGC

Q7027

AGCCTCAT

Q7028

GATTCTGC

Q7029

TCGTAGTG

Q7030

CTACGACA

Q7035

ATGGCATG

Q7036

GCAATGCA

Q7039

CTTATCGG

Q7040

TCCGCTAA

Q7041

GATCTATC

Q7042

AGCTCGCT

Q7047

ACACTAAG

Q7048

GTGTCGGA

AATGATACGGCGACCACCGAGATCTACAC[i5]TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG

Refer to Index 2 (i5) Orientation for more information on how to enter i5 bases on the sample sheet in forward or reverse complement orientation.

i5 Index Name

i5 Bases for Sample Sheet in Forward Orientation

i5 Bases for Sample Sheet in Reverse Complement Orientation

Q5001

AGCGCTAG

CTAGCGCT

Q5002

GATATCGA

TCGATATC

Q5003

CGCAGACG

CGTCTGCG

Q5004

TATGAGTA

TACTCATA

Q5007

ACATAGCG

CGCTATGT

Q5008

GTGCGATA

TATCGCAC

Q5009

CCAACAGA

TCTGTTGG

Q5010

TTGGTGAG

CTCACCAA

Q5013

AACCGCGG

CCGCGGTT

Q5014

GGTTATAA

TTATAACC

Q5017

CTAGCTTG

CAAGCTAG

Q5018

TCGATCCA

TGGATCGA

Q5025

ATACCAAG

CTTGGTAT

Q5026

GCGTTGGA

TCCAACGC

Q5027

CTTCACGG

CCGTGAAG

Q5028

TCCTGTAA

TTACAGGA

Q5029

CCTCGGTA

TACCGAGG

Q5030

TTCTAACG

CGTTAGAA

Q5031

CGCTCGTG

CACGAGCG

Q5032

TATCTACA

TGTAGATA

Q5035

CATTGTTG

CAACAATG

Q5036

TGCCACCA

TGGTGGCA

Q5039

ACGCCGCA

TGCGGCGT

Q5040

GTATTATG

CATAATAC