TruSeq Ribo Profile
Adapter Trimming
The following sequence is used for adapter trimming.
AGATCGGAAGAGCACACGTCT
3′ Adapter
5′ AGATCGGAAGAGCACACGTCT
Forward PCR Primer
5′ ATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACG
Index PCR Primers
5′ CAAGCAGAAGACGGCATACGAGAT[6 bases]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
Index Adapters
|
i7 Index Name |
Six-Base Sequence for Sample Sheet |
|---|---|
|
A001 |
ATCACG |
|
A002 |
CGATGT |
|
A003 |
TTAGGC |
|
A004 |
TGACCA |
|
A005 |
ACAGTG |
|
A006 |
GCCAAT |
|
A007 |
CAGATC |
|
A008 |
ACTTGA |
|
A009 |
GATCAG |
|
A010 |
TAGCTT |
|
A011 |
GGCTAC |
|
A012 |
CTTGTA |
