Nextera DNA Indexes

The i7 index names vary by kit:

•

H7xx—Nextera DNA CD Indexes (combinatorial dual)

•

N7xx—Nextera XT Index Kit v2, Nextera Index Kit

Index 2 (i5) Adapters

The i5 index names vary by kit:

•

E5xx—Nextera Rapid Capture Custom Enrichment Kit

•

H5xx—Nextera DNA CD Indexes (combinatorial dual)

•

N5xx—Nextera Index Kit

•

S5xx—Nextera XT Index Kit v2

Refer to Index 2 (i5) Orientation for more information on how to enter i5 bases on the sample sheet in forward or reverse complement orientation.

i5 Index Name	Bases in Adapter	i5 Bases for Sample Sheet in Forward Orientation	i5 Bases for Sample Sheet in Reverse Complement Orientation
[E/H/N/S]501	TAGATCGC	TAGATCGC	GCGATCTA
[E/H/N/S]502	CTCTCTAT	CTCTCTAT	ATAGAGAG
[E/H/N/S]503	TATCCTCT	TATCCTCT	AGAGGATA
[E/H/N/S]504	AGAGTAGA	AGAGTAGA	TCTACTCT
[E/H/N/S]505	GTAAGGAG	GTAAGGAG	CTCCTTAC
[E/H/N/S]506	ACTGCATA	ACTGCATA	TATGCAGT
[E/H/N/S]507	AAGGAGTA	AAGGAGTA	TACTCCTT
[E/H/N/S]508	CTAAGCCT	CTAAGCCT	AGGCTTAG
[E/H/N/S]510	CGTCTAAT	CGTCTAAT	ATTAGACG
[E/H/N/S]511	TCTCTCCG	TCTCTCCG	CGGAGAGA
[E/H/N/S]513	TCGACTAG	TCGACTAG	CTAGTCGA
[E/H/N/S]515	TTCTAGCT	TTCTAGCT	AGCTAGAA
[E/H/N/S]516	CCTAGAGT	CCTAGAGT	ACTCTAGG
[E/H/N/S]517	GCGTAAGA	GCGTAAGA	TCTTACGC
[E/H/N/S]518	CTATTAAG	CTATTAAG	CTTAATAG
[E/H/N/S]520	AAGGCTAT	AAGGCTAT	ATAGCCTT
[E/H/N/S]521	GAGCCTTA	GAGCCTTA	TAAGGCTC
[E/H/N/S]522	TTATGCGA	TTATGCGA	TCGCATAA