Sequences for Nextera, Illumina Prep, and Illumina PCR Kits

The following sequence is used for Read 1 and Read 2 adapter trimming.

CTGTCTCTTATACACATCT

The following sequence includes two adapter sequences joined by a plus sign. When performing adapter trimming, the software independently assesses each adapter for trimming.

CTGTCTCTTATACACATCT+ATGTGTATAAGAGACA

The following sequence includes two adapter sequences joined by a plus sign. When performing adapter trimming, the software independently assesses each adapter for trimming.

CTGTCTCTTATACACATCT+AGATGTGTATAAGAGACAG

The following transposase adapters are used for Nextera tagmentation.

Read 1

5′ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG

Read 2

5′ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG

Index 1 Read

5′ CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGG

Index 2 Read

5′ AATGATACGGCGACCACCGAGATCTACAC[i5]TCGTCGGCAGCGTC