Revision History

Document

Date

Description of Change

Document # 1000000002694 v19

October 2023

Corrected sequences for the following Illumina Unique Dual Indexes, Set A:

• UDP0069V3, UDP0070V3, UDP0071V3, UDP0072V3, UDP0073V3, UDP0074V3, UDP0075V3, UDP0076V3.

Added clarification for TSO 500 ctDNA v2 and TSO 500 HT kits.

Document # 1000000002694 v18

September 2023

Added adapter sequences for Illumina Unique Dual Indexes Set A-D and Illumina Unique Dual Indexes, LT.

Added clarification for entering the Index 2 (i5) bases on the sample sheet in forward or reverse orientation.

Added clarification of adapter trimming sequences usage.

Added clarification for V2 indexes used with IDT for Illumina UD Indexes Set C and Set D.

Document # 1000000002694 v17

February 2023

Updated references to NextSeq to include NextSeq 1000.

Added NovaSeq X Series to the i5 sample sheet v2.

For IDT for Illumina Indexes, Plate D/Set 4, corrected UDP0289, UDP0290, UDP0291, and UDP0301 sequences for i5 bases for sample sheets of the following instruments:

• iSeq 100
• NovaSeq 6000 with v1.5 reagent kits
• MiniSeq
• NextSeq 500/550
• HiSeq 3000/4000/X
• NextSeq 1000/2000 (sample sheet v1)

Added A-tailing information for TruSeq workflows.

Added information on the workflows used to prime and read the i5 index (Index 2).

Added adapter trimming sequences for TruSight DNA Enrichment, TruSight Tumor 170 and TruSight Oncology 500, TruSight Oncology ctDNA, and TruSight RNA Pan-Cancer Panel kits.

Document # 1000000002694 v16

April 2021

Added HTML format.

Document # 1000000002694 v15

February 2021

Corrected i7 bases in adapters sequences for TruSeq indexes.

Added the following sequences and bases:

• V1 indexes for UDI0015, UDI0016, UDI0055, UDI0056, UDP0252, UDP0258, UDP0289, UDP0290, UDP0291, and UDP0301.
• TruSight Tumor 15 i7 indexes.
• i5 bases in adapters for IDT for Illumina UD Indexes.

Document # 1000000002694 v14

July 2020

Added information in support of the NovaSeq 6000 Reagent Kit v1.5.

Added adapter sequencing for IDT for Illumina-PCR UD Indexes Set 1, 2, 3, and 4.

Document # 1000000002694 v13

June 2020

Replaced UDI0015, UDI0016, UDI0055, UDI0056, UDP0252, UDP0258, UDP0289, UDP0290, UDP0291, and UDP0301 with V2 versions.

Updated Nextera section to reflect new kit names.

Added NextSeq 2000 to the IDT for Illumina–TruSeq DNA and RNA UD Indexes table.

Document # 1000000002694 v12

March 2020

Added information for HiSeq X.

Added information for NextSeq 2000.

Added TruSight Oncology 500 adapter sequences.

Corrected the following information:

• IDT for Illumina Nextera DNA UD Indexes, Plate D adapter sequences for i5 bases iSeq, MiniSeq, NextSeq, HiSeq 3000/4000.
• TruSight Tumor 170 UP08 i7 and i5 index names.
• TruSight Tumor 170 UP08 and UP09 i7 adapter sequences.

Document # 1000000002694 v11

April 2019

Added adapter sequences for IDT for Illumina Nextera DNA UD Indexes Sets B, C, and D.

Document # 1000000002694 v10

February 2019

Added sequences for AmpliSeq UD Indexes for Illumina and AmpliSeq CD Indexes.

Renamed the following sections to include RNA:

• IDT for Illumina TruSeq DNA and RNA UD Indexes
• TruSeq DNA and RNA CD Indexes

Corrected TruSeq Small RNA sequences needed for sample sheet.

Document # 1000000002694 v09

November 2018

Updated the document structure:

• Consolidated sections by kit.
• Consolidated index adapter tables for TruSight DNA Enrichment and Nextera DNA indexes.
• Divided the IDT for Illumina UD Indexes section between the Nextera and TruSeq sections.
• Reorganized TruSight RNA Pan-Cancer Panel information for clarity and consistency.
• Reorganized TruSeq Small RNA index adapters into a table.
• Moved TruSeq Synthetic Long-Read DNA, TruSeq DNA Methylation, and TruSeq Ribo Profile sequences to Legacy Kits.

Added the following sequences and bases:

• Adapter trimming sequences where applicable.
• Bases for [E/H/N/S]517, a Nextera DNA i5 adapter.
• The i7 bases in adapter for TruSeq UD Indexes.
• The universal adapter sequence for TruSeq DNA Methylation.
• For TruSight Tumor 170, the i5 sample sheet bases for systems that do not require an i5 reverse complement.

Added the following miscellaneous information:

• The adapter sequences for TruSight RNA Pan-Cancer Panel and TruSeq Single Indexes can vary.
• Current versions of Sequencing Analysis Viewer do not show metrics for control sequences

Document # 1000000002694 v08

October 2018

Added IDT for Illumina Nextera DNA UD Indexes.

Document # 1000000002694 v07

June 2018

Added the iSeq 100 Sequencing System, which requires a reverse complement.

Document # 1000000002694 v06

February 2018

Added TruSight Tumor 170 indexes.

Document # 1000000002694 v05

February 2018

Updated IDT for Illumina to include 96 indexes.

Document # 1000000002694 v04

January 2018

Added AmpliSeq for Illumina Panels.

Document # 1000000002694 v03

October 2017

Corrected i5 bases for Nextera DNA CD Indexes for use with MiSeq and HiSeq systems.

Reorganized TruSeq sections.

Document # 1000000002694 v02

September 2017

Added adapters for Nextera DNA CD Indexes.

Document # 1000000002694 v01

February 2016

Added explanation of reverse complements in the sample sheet.

Corrected i5 adapter names for TruSight One to E502–E505.

Added adapters for TruSight RNA Pan-Cancer, TruSeq DNA Methylation, and TruSeq Ribo Profile.

Added MiniSeq, which requires a reverse complement.

Document # 1000000002694 v00

October 2015

Added information for the following TruSight kits:

• TruSight Cardio
• TruSight Myeloid Sequencing Panel
• TruSight One
• TruSight Rapid Capture
• TruSight Tumor 15
• TruSight Tumor 26

Created a TruSeq Amplicon section with information for the following kits:

• TruSeq Custom Amplicon 1.5
• TruSeq Amplicon Cancer Panel
• TruSeq Custom Amplicon Low Input

Marked obsolete kits as obsolete.

Grouped legacy kit information in new section titled Legacy Kits.

Reformatted and reorganized the contents.

Assigned document # 1000000002694.