Revision History

Added:

Added Index 2 base orientation information for MiSeq i100 Series.

Removed statements indicating that the read orientation of the Index 2 sequence is dictated by whether the Index 2 primer is grafted to the flow cell or in solution.

Corrected sequences for the following Illumina Unique Dual Indexes, Set A:

Added clarification for TSO 500 ctDNA v2 and TSO 500 HT kits.

Added adapter sequences for Illumina Unique Dual Indexes Set A-D and Illumina Unique Dual Indexes, LT.

Added clarification for entering the Index 2 (i5) bases on the sample sheet in forward or reverse orientation.

Added clarification of adapter trimming sequences usage.

Added clarification for V2 indexes used with IDT for Illumina UD Indexes Set C and Set D.

Updated references to NextSeq to include NextSeq 1000.

Added NovaSeq X Series to the i5 sample sheet v2.

For IDT for Illumina Indexes, Plate D/Set 4, corrected UDP0289, UDP0290, UDP0291, and UDP0301 sequences for i5 bases for sample sheets of the following instruments:

Added A-tailing information for TruSeq workflows.

Added information on the workflows used to prime and read the i5 index (Index 2).

Added adapter trimming sequences for TruSight DNA Enrichment, TruSight Tumor 170 and TruSight Oncology 500, TruSight Oncology ctDNA, and TruSight RNA Pan-Cancer Panel kits.

Added HTML format.

Corrected i7 bases in adapters sequences for TruSeq indexes.

Added the following sequences and bases:

Added information in support of the NovaSeq 6000 Reagent Kit v1.5.

Added adapter sequencing for IDT for Illumina-PCR UD Indexes Set 1, 2, 3, and 4.

Replaced UDI0015, UDI0016, UDI0055, UDI0056, UDP0252, UDP0258, UDP0289, UDP0290, UDP0291, and UDP0301 with V2 versions.

Updated Nextera section to reflect new kit names.

Added NextSeq 2000 to the IDT for Illumina–TruSeq DNA and RNA UD Indexes table.

Added information for HiSeq X.

Added information for NextSeq 2000.

Added TruSight Oncology 500 adapter sequences.

Corrected the following information:

Added adapter sequences for IDT for Illumina Nextera DNA UD Indexes Sets B, C, and D.

Added sequences for AmpliSeq UD Indexes for Illumina and AmpliSeq CD Indexes.

Renamed the following sections to include RNA:

Corrected TruSeq Small RNA sequences needed for sample sheet.

Updated the document structure:

Added the following sequences and bases:

Added the following miscellaneous information:

Added IDT for Illumina Nextera DNA UD Indexes.

Added the iSeq 100 Sequencing System, which requires a reverse complement.

Added TruSight Tumor 170 indexes.

Updated IDT for Illumina to include 96 indexes.

Added AmpliSeq for Illumina Panels.

Corrected i5 bases for Nextera DNA CD Indexes for use with MiSeq and HiSeq systems.

Reorganized TruSeq sections.

Added adapters for Nextera DNA CD Indexes.

Added explanation of reverse complements in the sample sheet.

Corrected i5 adapter names for TruSight One to E502–E505.

Added adapters for TruSight RNA Pan-Cancer, TruSeq DNA Methylation, and TruSeq Ribo Profile.

Added MiniSeq, which requires a reverse complement.