TruSeq DNA and RNA CD Indexes
Combinatorial dual (CD) index adapters (formerly TruSeq HT).
A-tailing is performed before adapter ligation. For example, the additional A base is in parentheses in the i7 adapter, as follows.
Index 1 (i7) Adapters
(A)GATCGGAAGAGCACACGTCTGAACTCCAGTCAC[i7]ATCTCGTATGCCGTCTTCTGCTTG
Adapter Trimming
The following sequences are used for adapter trimming.
Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA
Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
Index 1 (i7) Adapters
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC[i7]ATCTCGTATGCCGTCTTCTGCTTG
|
i7 Index Name |
i7 Bases for Sample Sheet |
|---|---|
|
D701 |
ATTACTCG |
|
D702 |
TCCGGAGA |
|
D703 |
CGCTCATT |
|
D704 |
GAGATTCC |
|
D705 |
ATTCAGAA |
|
D706 |
GAATTCGT |
|
D707 |
CTGAAGCT |
|
D708 |
TAATGCGC |
|
D709 |
CGGCTATG |
|
D710 |
TCCGCGAA |
|
D711 |
TCTCGCGC |
|
D712 |
AGCGATAG |
Index 2 (i5) Adapters
AATGATACGGCGACCACCGAGATCTACAC[i5]ACACTCTTTCCCTACACGACGCTCTTCCGATCT
Refer to Index 2 (i5) Orientation for more information on how to enter i5 bases on the sample sheet in forward or reverse complement orientation.
|
i5 Index Name |
i5 Bases for Sample Sheet in Forward Orientation |
i5 Bases for Sample Sheet in Reverse Complement Orientation |
|---|---|---|
|
D501 |
TATAGCCT |
AGGCTATA |
|
D502 |
ATAGAGGC |
GCCTCTAT |
|
D503 |
CCTATCCT |
AGGATAGG |
|
D504 |
GGCTCTGA |
TCAGAGCC |
|
D505 |
AGGCGAAG |
CTTCGCCT |
|
D506 |
TAATCTTA |
TAAGATTA |
|
D507 |
CAGGACGT |
ACGTCCTG |
|
D508 |
GTACTGAC |
GTCAGTAC |
