IDT for Illumina–TruSeq DNA and RNA UD Indexes

The IDT for Illumina TruSeq unique dual (UD) index adapters are arranged in the plate to enforce the recommended pairing strategy.

A-tailing is performed before adapter ligation. For example, the additional A base is in parentheses in the i7 adapter, as follows.

Index 1 (i7) Adapters

(A)GATCGGAAGAGCACACGTCTGAACTCCAGTCAC[i7]ATCTCGTATGCCGTCTTCTGCTTG

Index Adapters

Index 1 (i7) Adapters

GATCGGAAGAGCACACGTCTGAACTCCAGTCAC[i7]ATCTCGTATGCCGTCTTCTGCTTG

Index 2 (i5) Adapters

AATGATACGGCGACCACCGAGATCTACAC[i5]ACACTCTTTCCCTACACGACGCTCTTCCGATCT

Refer to Index 2 (i5) Orientation for more information on how to enter i5 bases on the sample sheet in forward or reverse complement orientation.

Index Name	i7 Bases in Adapter	i7 Bases for Sample Sheet	i5 Bases in Adapter	i5 Bases for Sample Sheet in Forward Orientation	i5 Bases for Sample Sheet in Reverse Complement Orientation
UDI0001	CCGCGGTT	CCGCGGTT	AGCGCTAG	AGCGCTAG	CTAGCGCT
UDI0002	TTATAACC	TTATAACC	GATATCGA	GATATCGA	TCGATATC
UDI0003	GGACTTGG	GGACTTGG	CGCAGACG	CGCAGACG	CGTCTGCG
UDI0004	AAGTCCAA	AAGTCCAA	TATGAGTA	TATGAGTA	TACTCATA
UDI0005	ATCCACTG	ATCCACTG	AGGTGCGT	AGGTGCGT	ACGCACCT
UDI0006	GCTTGTCA	GCTTGTCA	GAACATAC	GAACATAC	GTATGTTC
UDI0007	CAAGCTAG	CAAGCTAG	ACATAGCG	ACATAGCG	CGCTATGT
UDI0008	TGGATCGA	TGGATCGA	GTGCGATA	GTGCGATA	TATCGCAC
UDI0009	AGTTCAGG	AGTTCAGG	CCAACAGA	CCAACAGA	TCTGTTGG
UDI0010	GACCTGAA	GACCTGAA	TTGGTGAG	TTGGTGAG	CTCACCAA
UDI0011	TCTCTACT	TCTCTACT	CGCGGTTC	CGCGGTTC	GAACCGCG
UDI0012	CTCTCGTC	CTCTCGTC	TATAACCT	TATAACCT	AGGTTATA
UDI0013	CCAAGTCT	CCAAGTCT	AAGGATGA	AAGGATGA	TCATCCTT
UDI0014	TTGGACTC	TTGGACTC	GGAAGCAG	GGAAGCAG	CTGCTTCC
UDI0015V2	CAGTAGGC	CAGTAGGC	TGACGAAT	TGACGAAT	ATTCGTCA
UDI0015	GGCTTAAG	GGCTTAAG	TCGTGACC	TCGTGACC	GGTCACGA
UDI0016V2	TGACGAAT	TGACGAAT	CAGTAGGC	CAGTAGGC	GCCTACTG
UDI0016	AATCCGGA	AATCCGGA	CTACAGTT	CTACAGTT	AACTGTAG
UDI0017	TAATACAG	TAATACAG	ATATTCAC	ATATTCAC	GTGAATAT
UDI0018	CGGCGTGA	CGGCGTGA	GCGCCTGT	GCGCCTGT	ACAGGCGC
UDI0019	ATGTAAGT	ATGTAAGT	ACTCTATG	ACTCTATG	CATAGAGT
UDI0020	GCACGGAC	GCACGGAC	GTCTCGCA	GTCTCGCA	TGCGAGAC
UDI0021	GGTACCTT	GGTACCTT	AAGACGTC	AAGACGTC	GACGTCTT
UDI0022	AACGTTCC	AACGTTCC	GGAGTACT	GGAGTACT	AGTACTCC
UDI0023	GCAGAATT	GCAGAATT	ACCGGCCA	ACCGGCCA	TGGCCGGT
UDI0024	ATGAGGCC	ATGAGGCC	GTTAATTG	GTTAATTG	CAATTAAC
UDI0025	ACTAAGAT	ACTAAGAT	AACCGCGG	AACCGCGG	CCGCGGTT
UDI0026	GTCGGAGC	GTCGGAGC	GGTTATAA	GGTTATAA	TTATAACC
UDI0027	CTTGGTAT	CTTGGTAT	CCAAGTCC	CCAAGTCC	GGACTTGG
UDI0028	TCCAACGC	TCCAACGC	TTGGACTT	TTGGACTT	AAGTCCAA
UDI0029	CCGTGAAG	CCGTGAAG	CAGTGGAT	CAGTGGAT	ATCCACTG
UDI0030	TTACAGGA	TTACAGGA	TGACAAGC	TGACAAGC	GCTTGTCA
UDI0031	GGCATTCT	GGCATTCT	CTAGCTTG	CTAGCTTG	CAAGCTAG
UDI0032	AATGCCTC	AATGCCTC	TCGATCCA	TCGATCCA	TGGATCGA
UDI0033	TACCGAGG	TACCGAGG	CCTGAACT	CCTGAACT	AGTTCAGG
UDI0034	CGTTAGAA	CGTTAGAA	TTCAGGTC	TTCAGGTC	GACCTGAA
UDI0035	AGCCTCAT	AGCCTCAT	AGTAGAGA	AGTAGAGA	TCTCTACT
UDI0036	GATTCTGC	GATTCTGC	GACGAGAG	GACGAGAG	CTCTCGTC
UDI0037	TCGTAGTG	TCGTAGTG	AGACTTGG	AGACTTGG	CCAAGTCT
UDI0038	CTACGACA	CTACGACA	GAGTCCAA	GAGTCCAA	TTGGACTC
UDI0039	TAAGTGGT	TAAGTGGT	CTTAAGCC	CTTAAGCC	GGCTTAAG
UDI0040	CGGACAAC	CGGACAAC	TCCGGATT	TCCGGATT	AATCCGGA
UDI0041	ATATGGAT	ATATGGAT	CTGTATTA	CTGTATTA	TAATACAG
UDI0042	GCGCAAGC	GCGCAAGC	TCACGCCG	TCACGCCG	CGGCGTGA
UDI0043	AAGATACT	AAGATACT	ACTTACAT	ACTTACAT	ATGTAAGT
UDI0044	GGAGCGTC	GGAGCGTC	GTCCGTGC	GTCCGTGC	GCACGGAC
UDI0045	ATGGCATG	ATGGCATG	AAGGTACC	AAGGTACC	GGTACCTT
UDI0046	GCAATGCA	GCAATGCA	GGAACGTT	GGAACGTT	AACGTTCC
UDI0047	GTTCCAAT	GTTCCAAT	AATTCTGC	AATTCTGC	GCAGAATT
UDI0048	ACCTTGGC	ACCTTGGC	GGCCTCAT	GGCCTCAT	ATGAGGCC
UDI0049	ATATCTCG	ATATCTCG	ATCTTAGT	ATCTTAGT	ACTAAGAT
UDI0050	GCGCTCTA	GCGCTCTA	GCTCCGAC	GCTCCGAC	GTCGGAGC
UDI0051	AACAGGTT	AACAGGTT	ATACCAAG	ATACCAAG	CTTGGTAT
UDI0052	GGTGAACC	GGTGAACC	GCGTTGGA	GCGTTGGA	TCCAACGC
UDI0053	CAACAATG	CAACAATG	CTTCACGG	CTTCACGG	CCGTGAAG
UDI0054	TGGTGGCA	TGGTGGCA	TCCTGTAA	TCCTGTAA	TTACAGGA
UDI0055V2	GTTCGCCG	GTTCGCCG	GCTCATTG	GCTCATTG	CAATGAGC
UDI0055	AGGCAGAG	AGGCAGAG	AGAATGCC	AGAATGCC	GGCATTCT
UDI0056V2	CACGAGCG	CACGAGCG	ATCTGCCA	ATCTGCCA	TGGCAGAT
UDI0056	GAATGAGA	GAATGAGA	GAGGCATT	GAGGCATT	AATGCCTC
UDI0057	TGCGGCGT	TGCGGCGT	CCTCGGTA	CCTCGGTA	TACCGAGG
UDI0058	CATAATAC	CATAATAC	TTCTAACG	TTCTAACG	CGTTAGAA
UDI0059	GATCTATC	GATCTATC	ATGAGGCT	ATGAGGCT	AGCCTCAT
UDI0060	AGCTCGCT	AGCTCGCT	GCAGAATC	GCAGAATC	GATTCTGC
UDI0061	CGGAACTG	CGGAACTG	CACTACGA	CACTACGA	TCGTAGTG
UDI0062	TAAGGTCA	TAAGGTCA	TGTCGTAG	TGTCGTAG	CTACGACA
UDI0063	TTGCCTAG	TTGCCTAG	ACCACTTA	ACCACTTA	TAAGTGGT
UDI0064	CCATTCGA	CCATTCGA	GTTGTCCG	GTTGTCCG	CGGACAAC
UDI0065	ACACTAAG	ACACTAAG	ATCCATAT	ATCCATAT	ATATGGAT
UDI0066	GTGTCGGA	GTGTCGGA	GCTTGCGC	GCTTGCGC	GCGCAAGC
UDI0067	TTCCTGTT	TTCCTGTT	AGTATCTT	AGTATCTT	AAGATACT
UDI0068	CCTTCACC	CCTTCACC	GACGCTCC	GACGCTCC	GGAGCGTC
UDI0069	GCCACAGG	GCCACAGG	CATGCCAT	CATGCCAT	ATGGCATG
UDI0070	ATTGTGAA	ATTGTGAA	TGCATTGC	TGCATTGC	GCAATGCA
UDI0071	ACTCGTGT	ACTCGTGT	ATTGGAAC	ATTGGAAC	GTTCCAAT
UDI0072	GTCTACAC	GTCTACAC	GCCAAGGT	GCCAAGGT	ACCTTGGC
UDI0073	CAATTAAC	CAATTAAC	CGAGATAT	CGAGATAT	ATATCTCG
UDI0074	TGGCCGGT	TGGCCGGT	TAGAGCGC	TAGAGCGC	GCGCTCTA
UDI0075	AGTACTCC	AGTACTCC	AACCTGTT	AACCTGTT	AACAGGTT
UDI0076	GACGTCTT	GACGTCTT	GGTTCACC	GGTTCACC	GGTGAACC
UDI0077	TGCGAGAC	TGCGAGAC	CATTGTTG	CATTGTTG	CAACAATG
UDI0078	CATAGAGT	CATAGAGT	TGCCACCA	TGCCACCA	TGGTGGCA
UDI0079	ACAGGCGC	ACAGGCGC	CTCTGCCT	CTCTGCCT	AGGCAGAG
UDI0080	GTGAATAT	GTGAATAT	TCTCATTC	TCTCATTC	GAATGAGA
UDI0081	AACTGTAG	AACTGTAG	ACGCCGCA	ACGCCGCA	TGCGGCGT
UDI0082	GGTCACGA	GGTCACGA	GTATTATG	GTATTATG	CATAATAC
UDI0083	CTGCTTCC	CTGCTTCC	GATAGATC	GATAGATC	GATCTATC
UDI0084	TCATCCTT	TCATCCTT	AGCGAGCT	AGCGAGCT	AGCTCGCT
UDI0085	AGGTTATA	AGGTTATA	CAGTTCCG	CAGTTCCG	CGGAACTG
UDI0086	GAACCGCG	GAACCGCG	TGACCTTA	TGACCTTA	TAAGGTCA
UDI0087	CTCACCAA	CTCACCAA	CTAGGCAA	CTAGGCAA	TTGCCTAG
UDI0088	TCTGTTGG	TCTGTTGG	TCGAATGG	TCGAATGG	CCATTCGA
UDI0089	TATCGCAC	TATCGCAC	CTTAGTGT	CTTAGTGT	ACACTAAG
UDI0090	CGCTATGT	CGCTATGT	TCCGACAC	TCCGACAC	GTGTCGGA
UDI0091	GTATGTTC	GTATGTTC	AACAGGAA	AACAGGAA	TTCCTGTT
UDI0092	ACGCACCT	ACGCACCT	GGTGAAGG	GGTGAAGG	CCTTCACC
UDI0093	TACTCATA	TACTCATA	CCTGTGGC	CCTGTGGC	GCCACAGG
UDI0094	CGTCTGCG	CGTCTGCG	TTCACAAT	TTCACAAT	ATTGTGAA
UDI0095	TCGATATC	TCGATATC	ACACGAGT	ACACGAGT	ACTCGTGT
UDI0096	CTAGCGCT	CTAGCGCT	GTGTAGAC	GTGTAGAC	GTCTACAC