TruSight Tumor 170 and TruSight Oncology 500
Adapter Trimming
The following sequences are used for adapter trimming.
Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA
Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
RNA Index 1 (i7) Adapters
|
i7 Index Name |
Index Primer |
i7 Bases for Sample Sheet |
|---|---|---|
|
D702 |
UP01 |
TCCGGAGA |
|
D707 |
UP02 |
CTGAAGCT |
|
D717 |
UP03 |
CGTAGCTC |
|
D706 |
UP04 |
GAATTCGT |
|
D712 |
UP05 |
AGCGATAG |
|
D724 |
UP06 |
GCGATTAA |
|
D705 |
UP07 |
ATTCAGAA |
|
D713 |
UP08 |
GAATAATC |
|
D715 |
UP09 |
TTAATCAG |
|
D703 |
UP10 |
CGCTCATT |
|
D710 |
UP11 |
TCCGCGAA |
|
D701 |
UP12 |
ATTACTCG |
|
D716 |
UP13 |
ACTGCTTA |
|
D714 |
UP14 |
ATGCGGCT |
|
D718 |
UP15 |
GCCTCTCT |
|
D719 |
UP16 |
GCCGTAGG |
RNA Index 2 (i5) Adapters
Refer to Index 2 (i5) Orientation for more information on how to enter i5 bases on the sample sheet in forward or reverse complement orientation.
|
i5 Index Name |
Index Primer |
i5 Bases for Sample Sheet in Forward Orientation |
i5 Bases for Sample Sheet in Reverse Complement Orientation |
|---|---|---|---|
|
D503 |
UP01 |
CCTATCCT |
AGGATAGG |
|
D504 |
UP02 |
GGCTCTGA |
TCAGAGCC |
|
D509 |
UP03 |
TTCGGATG |
CATCCGAA |
|
D510 |
UP04 |
ACTCATAA |
TTATGAGT |
|
D513 |
UP05 |
TTATTCGT |
ACGAATAA |
|
D515 |
UP06 |
AGCAGATC |
GATCTGCT |
|
D501 |
UP07 |
TATAGCCT |
AGGCTATA |
|
D502 |
UP08 |
ATAGAGGC |
GCCTCTAT |
|
D505 |
UP09 |
AGGCGAAG |
CTTCGCCT |
|
D506 |
UP10 |
TAATCTTA |
TAAGATTA |
|
D517 |
UP11 |
TACTTACT |
AGTAAGTA |
|
D518 |
UP12 |
AGGAAGTC |
GACTTCCT |
|
D511 |
UP13 |
GCGCCTCT |
AGAGGCGC |
|
D512 |
UP14 |
CGCGGCTA |
TAGCCGCG |
|
D514 |
UP15 |
CCTACGAA |
TTCGTAGG |
|
D516 |
UP16 |
GCGGAGCG |
CGCTCCGC |
DNA Index 1 (i7) Adapters
|
i7 Index Name |
Index Primer |
i7 Bases for Sample Sheet |
|---|---|---|
|
D721 |
CP01 |
CATCGAGG |
|
D723 |
CP02 |
CTCGACTG |
|
D709 |
CP03 |
CGGCTATG |
|
D711 |
CP04 |
TCTCGCGC |
|
D723 |
CP05 |
CTCGACTG |
|
D709 |
CP06 |
CGGCTATG |
|
D711 |
CP07 |
TCTCGCGC |
|
D721 |
CP08 |
CATCGAGG |
|
D709 |
CP09 |
CGGCTATG |
|
D711 |
CP10 |
TCTCGCGC |
|
D721 |
CP11 |
CATCGAGG |
|
D723 |
CP12 |
CTCGACTG |
|
D711 |
CP13 |
TCTCGCGC |
|
D721 |
CP14 |
CATCGAGG |
|
D723 |
CP15 |
CTCGACTG |
|
D709 |
CP16 |
CGGCTATG |
DNA Index 2 (i5) Adapters
Refer to Index 2 (i5) Orientation for more information on how to enter i5 bases on the sample sheet in forward or reverse complement orientation.
|
i5 Index Name |
Index Primer |
i5 Bases for Sample Sheet in Forward Orientation |
i5 Bases for Sample Sheet in Reverse Complement Orientation |
|---|---|---|---|
|
D507 |
CP01 |
CAGGACGT |
ACGTCCTG |
|
D508 |
CP02 |
GTACTGAC |
GTCAGTAC |
|
D519 |
CP03 |
GGCGACGG |
CCGTCGCC |
|
D520 |
CP04 |
CCTCGGAC |
GTCCGAGG |
|
D507 |
CP05 |
CAGGACGT |
ACGTCCTG |
|
D507 |
CP06 |
CAGGACGT |
ACGTCCTG |
|
D507 |
CP07 |
CAGGACGT |
ACGTCCTG |
|
D508 |
CP08 |
GTACTGAC |
GTCAGTAC |
|
D508 |
CP09 |
GTACTGAC |
GTCAGTAC |
|
D508 |
CP10 |
GTACTGAC |
GTCAGTAC |
|
D519 |
CP11 |
GGCGACGG |
CCGTCGCC |
|
D519 |
CP12 |
GGCGACGG |
CCGTCGCC |
|
D519 |
CP13 |
GGCGACGG |
CCGTCGCC |
|
D520 |
CP14 |
CCTCGGAC |
GTCCGAGG |
|
D520 |
CP15 |
CCTCGGAC |
GTCCGAGG |
|
D520 |
CP16 |
CCTCGGAC |
GTCCGAGG |
