TruSeq DNA Methylation
Adapter Trimming
The following sequence is used for adapter trimming.
Read 1
AGATCGGAAGAGCACACGTCTGAAC
Read 2
AGATCGGAAGAGCGTCGTGTAGGGA
TruSeq Universal Adapter
5′ AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
Index PCR Primers
5′ CAAGCAGAAGACGGCATACGAGAT[6 bases]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
Index Adapters
|
Index Name |
Six-Base Sequence for Sample Sheet |
|---|---|
|
Index 1 |
ATCACG |
|
Index 2 |
CGATGT |
|
Index 3 |
TTAGGC |
|
Index 4 |
TGACCA |
|
Index 5 |
ACAGTG |
|
Index 6 |
GCCAAT |
|
Index 7 |
CAGATC |
|
Index 8 |
ACTTGA |
|
Index 9 |
GATCAG |
|
Index 10 |
TAGCTT |
|
Index 11 |
GGCTAC |
|
Index 12 |
CTTGTA |
