Common Clustering Issues and Prevention

Clustering issues occur when a patterned flow cell is loaded with too high a concentration (overloading) or too low a concentration (underloading) for effective sequencing. For nonpatterened flow cells, too many clusters (overclustering) or too few clusters (underclustering) create these issues.

Inconsistent clustering from run to run or across a flow cell can indicate incompatibility between the library and system.