Diagnosing Suboptimal Clustering (Nonpatterned Flow Cells)

During a run, monitor certain run metrics and thumbnail images to diagnose overclustering of a nonpatterned flow cell. Diagnosing overclustering early, canceling runs when necessary, or quickly identifying the root cause of a run failure improves sequencing efficiency.

Use Run Metrics Software (Run Metrics Software) or Analysis Software (AS) to monitor run metrics. Run Metrics Software divides run metrics between several tabs, three of which are helpful for diagnosing overclustering. In BaseSpace Sequence Hub, the Charts and Metrics tabs under the Runs tab display the same metrics.

This section specifically describes how to use the software to diagnose overclustering. For more information on either application, see the Sequencing Analysis Viewer Software Guide (document # 15066069) or BaseSpace Sequence Hub Online Help.