ExAmp Reagent Preparation

For systems and workflows that require manually mixing ExAmp reagents, improper ExAmp preparation can impact metrics for percent occupancy and clusters passing filter. Percent occupancy indicates the percentage of wells on a patterned flow cell that contain at least one cluster, regardless of whether the clusters passed filer.

Follow instructions for preparing ExAmp reagents carefully, using the specified volumes and durations.

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ExAmp mixing—ExAmp reagents are viscous and must be mixed carefully. Pipette and dispense slowly so that the entire volume is expelled from the tip.

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ExAmp staging time—When too much time elapses between combining the ExAmp reagents and loading them onto the flow cell, the reagents start to degrade.

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Flow cell staging time (NovaSeq Xp workflow only)—When too much time elapses between loading the ExAmp/library mix onto the flow cell and starting the run, clusters form prematurely.