Library Quality

Library prep contaminants such as adapter dimers, primer dimers, or partial library constructs can impact quantification and clustering. Insufficient library cleanup can cause the presence of these contaminants.

Verify the quality and purity of all libraries using the method described in the library prep documentation, such as Bioanalyzer or Fragment Analyzer. Check for library integrity, average insert size, and contaminants. The average insert size is necessary for calculating library molarity.