Illumina Single Cell Prep UDI Mix Strip
Illumina Single Cell 3' RNA Prep libraries require a minimum of 1% PhiX to be added to the loaded library pools. It is recommended to increase PhiX to a minimum of 2% with the NovaSeq X Series.
The Illumina Single Cell Prep UDI Mix Strip uses the same set of indexes as the Illumina Single Cell Unique Dual Indexes plate. Do not use the plate and mix strip together.
|
Well |
Index Name |
|---|---|
|
Mix 1 |
SCUDP0017 |
|
Mix 2 |
SCUDP0018 |
|
Mix 3 |
SCUDP0019 |
|
Mix 4 |
SCUDP0020 |
|
Mix 5 |
SCUDP0021 |
|
Mix 6 |
SCUDP0022 |
|
Mix 7 |
SCUDP0023 |
|
Mix 8 |
SCUDP0024 |
≤ Four-Plex Pooling Strategies
The following table shows index adapters (wells) that can be combined in a two-plex through four-plex pool.
|
Plexity |
Combinations in UDI Mix Strip |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
2 |
|
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|
3 |
|
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|
4 |
|
[NovaSeq X Series or NextSeq 1000/2000 with XLEAP-SBS Chemistry] ≤ Four-Plex Pooling Strategies
This section applies to NovaSeq X Series or NextSeq 1000/2000 instruments using XLEAP-SBS Chemistry.
The following table shows index adapters (wells) that can be combined in a two-plex through four-plex pool.
|
Plexity |
Combinations in UDI Mix Strip |
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
2 |
|
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|
3 |
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|
4 |
|
