Nextera DNA CD Indexes (24 Indexes, 24 Samples)
Nextera DNA CD Indexes (24 indexes, 24 samples) contain six Index 1 (i7) adapters and four Index 2 (i5) adapters packaged in tubes. The following table shows strategies for pooling three to eight dual-indexed libraries prepared with these indexes.
A minimum plexity of three makes sure that libraries are color balanced for sequencing on any Illumina system. To create a two-plex pool, review the index adapter sequences to ensure color balance on your system.
|
Plexity |
Index 1 (i7) Adapters |
Index 2 (i5) Adapters |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
3 |
One of the following combinations:
|
H505, H506, and H517 |
||||||||||||
|
4 |
One of the following combinations:
|
H503, H505, H506, and H517 |
||||||||||||
|
5 |
One of the following combinations:
|
H503, H505, H506, H517, and any other i5 adapter |
||||||||||||
|
6 |
One of the following combinations:
|
One of the following combinations:
|
||||||||||||
|
7 |
One of the following combinations:
|
One of the following combinations:
|
||||||||||||
|
8 |
H705, H706, H707, H710, H711, H714, and two other i7 adapters |
H503, H505, H506, and H517, used two times each |
Four-Plex Example
Libraries with the following Index 1 and Index 2 adapter pairs can be combined in a four-plex pool:
| • | H705-H503 |
| • | H706-H505 |
| • | H707-H506 |
| • | H710-H517 |
Five-Plex Example
Libraries with the following Index 1 and Index 2 adapter pairs can be combined in a five-plex pool:
| • | H503-H705 |
| • | H505-H706 |
| • | H506-H707 |
| • | H517-H710 |
| • | H503-H711 |
Six-Plex Example
Libraries with the following Index 1 and Index 2 adapter pairs can be combined in a six-plex pool:
| • | H705-H503 |
| • | H706-H505 |
| • | H707-H506 |
| • | H710-H517 |
| • | H711-H503 |
| • | H714-H505 |
