Column-Based Strategies

The following table shows index adapters (wells) that can be combined in a two- to eight-plex pool. The color‑coded figure illustrates each combination.

All combinations apply to any column on the plate. The minimum plexity for a column is two.

Plexity

Combinations

Color in Figure

2

Two consecutive wells in a column:

A and B
C and D
E and F
G and H

Orange

3

Three consecutive wells in a column:

A–C
D–F
C–E
F–H

Gray

4

Four consecutive wells in a column:

A–D
E–H

Purple

5

Five consecutive wells in a column:

A–E
D–H

Blue

6

Six consecutive wells in a column:

A–F
C–H

Green

7

Seven consecutive wells in a column:

A–G
B–H

Pink

8

The entire column. All eight combinations are unique.

Teal