Illumina Single Cell Prep UDI Mix Strip

Illumina Single Cell 3' RNA Prep libraries require a minimum of 1% PhiX to be added to the loaded library pools. It is recommended to increase PhiX to a minimum of 2% with the NovaSeq X Series.

The Illumina Single Cell Prep UDI Mix Strip uses the same set of indexes as the Illumina Single Cell Unique Dual Indexes plate. Do not use the plate and mix strip together.

UDI Mix Strip Layout

Well

Index Name

Mix 1

SCUDP0017

Mix 2

SCUDP0018

Mix 3

SCUDP0019

Mix 4

SCUDP0020

Mix 5

SCUDP0021

Mix 6

SCUDP0022

Mix 7

SCUDP0023

Mix 8

SCUDP0024

Sample Name

Index Combination to Use

Instrument(s)

Sample 1

UDI Library Index mix 5

Only three-plex option for NovaSeq X Series

 

 

Sample 2

UDI Library Index mix 6

Sample 3

UDI Library Index mix 7

Sample 1

UDI Library Index mix 1

Three-plex option for NextSeq 2000

 

 

Sample 2

UDI Library Index mix 2

Sample 3

UDI Library Index mix 3

Sample 1

UDI Library Index mix 4

Three-plex option for NextSeq 2000

 

 

Sample 2

UDI Library Index mix 5

Sample 3

UDI Library Index mix 6

Sample 1

UDI Library Index mix 6

Three-plex option for NextSeq 2000

 

 

Sample 2

UDI Library Index mix 7

Sample 3

UDI Library Index mix 8

Sample 1

UDI Library Index mix 1

Four-plex option for NovaSeq X Series or NextSeq 2000

 

 

 

Sample 2

UDI Library Index mix 2

Sample 3

UDI Library Index mix 3

Sample 4

UDI Library Index mix 4

Sample 1

UDI Library Index mix 5

Four-plex option for NovaSeq X Series or NextSeq 2000

 

 

 

Sample 2

UDI Library Index mix 6

Sample 3

UDI Library Index mix 7

Sample 4

UDI Library Index mix 8