Nextera DNA CD Indexes (96 Indexes, 96 Samples)
The following table depicts the plate layout for Nextera DNA CD Indexes (96 indexes, 96 samples), which are designed for dual indexing.
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
A |
H701 H505 |
H702 H506 |
H703 H517 |
H705 H505 |
H707 H506 |
H723 H517 |
H706 H505 |
H712 H506 |
H720 H517 |
H710 H505 |
H711 H506 |
H714 H517 |
|
B |
H702 H517 |
H703 H505 |
H701 H506 |
H707 H517 |
H723 H505 |
H705 H506 |
H712 H517 |
H720 H505 |
H706 H506 |
H711 H517 |
H714 H505 |
H710 H506 |
|
C |
H703 H506 |
H701 H517 |
H702 H505 |
H723 H506 |
H705 H517 |
H707 H505 |
H720 H506 |
H706 H517 |
H712 H505 |
H714 H506 |
H710 H517 |
H711 H505 |
|
D |
H705 H503 |
H707 H503 |
H723 H503 |
H706 H503 |
H712 H503 |
H720 H503 |
H710 H503 |
H711 H503 |
H714 H503 |
H701 H503 |
H702 H503 |
H703 H503 |
|
E |
H706 H516 |
H712 H516 |
H720 H516 |
H710 H516 |
H711 H516 |
H714 H516 |
H701 H516 |
H702 H516 |
H703 H516 |
H705 H516 |
H707 H516 |
H723 H516 |
|
F |
H710 H522 |
H711 H510 |
H714 H513 |
H701 H522 |
H702 H510 |
H703 H513 |
H705 H522 |
H707 H510 |
H723 H513 |
H706 H522 |
H712 H510 |
H720 H513 |
|
G |
H711 H513 |
H714 H522 |
H710 H510 |
H702 H513 |
H703 H522 |
H701 H510 |
H707 H513 |
H723 H522 |
H705 H510 |
H712 H513 |
H720 H522 |
H706 H510 |
|
H |
H714 H510 |
H710 H513 |
H711 H522 |
H703 H510 |
H701 H513 |
H702 H522 |
H723 H510 |
H705 H513 |
H707 H522 |
H720 H510 |
H706 H513 |
H712 H522 |
Three-Plex Through Eight-Plex Pooling Strategies
The following table shows index adapters (wells) that can be combined in a three- to eight-plex pool, while the color‑coded figure illustrates each combination.
A minimum plexity of three makes sure that libraries are color balanced for sequencing on any Illumina system. To create a two-plex pool, review the index adapter sequences to ensure color balance on your system.
|
Plexity |
Combinations |
Color in Figure |
||||||
|---|---|---|---|---|---|---|---|---|
|
3 |
The first three or last three wells in a column:
Rows D and E are not used. |
Gray |
||||||
|
4 |
The first four or last four wells in a column:
|
Purple |
||||||
|
5 |
The first five or last five wells in a column:
|
Blue |
||||||
|
6 |
[Option 1] The first six or last six wells in a column:
[Option 2] The first two wells (A and B) or last two wells (G and H) |
Green |
||||||
|
7 |
The first seven or last seven wells in a column:
|
Pink |
||||||
|
8 |
The entire column. |
Teal |
Nine-Plex Pooling Strategies
Use index adapters from any wells that optimize color balance in a sequencing run, for example:
| • | A1–H1 and A2 |
| • | A4–D4 and A5–E5 |
| • | A7–F7 and A8–C8 |
| • | A10–C10, A11–C11, and A12–C12 |
The following figure depicts all four examples.
