Nextera XT Index Kit v2 and Nextera Index Kit

The Nextera XT Index Kit v2 is available in Sets A–D. Each set contains 12 Index 1 (i7) and eight Index 2 (i5) adapters packaged in tubes.

The Nextera Index Kit also packages Index 1 and Index 2 adapters in tubes, and is available in the following sizes:

• 24 indexes, 96 samples—Contains six Index 1 and four Index 2 adapters.
• 96 indexes, 384 samples—Contains 12 Index 1 adapters and eight Index 2 adapters.

The following table shows strategies for pooling dual-indexed libraries for dual-indexed sequencing, which sequences Index 1 and Index 2.

Plexity

Index 1 (i7) Adapters

Index 2 (i5) Adapters

2–6

At least two unique i7 adapters

At least two unique i5 adapters

7–12

One of the following combinations:

• N701, N702, N704, and any other i7 adapter
• N703, N705, N706, and any other i7 adapter

One of the following combinations:

• N503 and N504
• N505 and N506

> 12

N701–N706 and any other i7 adapter

One of the following combinations:

• N503, N504, and any other i5 adapter
• N505, N506, and any other i5 adapter

The following table shows strategies for creating 2–12-plex pools of dual-indexed libraries for single-indexed sequencing, which sequences Index 1 only.

Plexity

Index 1 (i7) Adapters

Index 2 (i5) Adapters

2

One of the following combinations:

• N701 and N702
• N702 and N704

Any i5 adapters

3

One of the following combinations:

• N701, N702, and N704
• N703, N705, and N706

Any i5 adapters

4 or 5

One of the following combinations:

• N701, N702, N704, and any other i7 adapter
• N703, N705, N706, and any other i7 adapter

Any i5 adapters

6

N701, N702, N703, N704, N705, and N706

Any i5 adapters

7–12

N701–N706 and any other i7 adapter

Any i5 adapters