TruSeq CD Indexes (Dual Indexing)
The following table depicts the plate layout for TruSeq DNA CD Indexes and TruSeq RNA CD Indexes (formerly TruSeq HT Indexes).
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
A |
D701 D501 |
D702 D501 |
D703 D501 |
D704 D501 |
D705 D501 |
D706 D501 |
D707 D501 |
D708 D501 |
D709 D501 |
D710 D501 |
D711 D501 |
D712 D501 |
|
B |
D701 D502 |
D702 D502 |
D703 D502 |
D704 D502 |
D705 D502 |
D706 D502 |
D707 D502 |
D708 D502 |
D709 D502 |
D710 D502 |
D711 D502 |
D712 D502 |
|
C |
D701 D503 |
D702 D503 |
D703 D503 |
D704 D503 |
D705 D503 |
D706 D503 |
D707 D503 |
D708 D503 |
D709 D503 |
D710 D503 |
D711 D503 |
D712 D503 |
|
D |
D701 D504 |
D702 D504 |
D703 D504 |
D704 D504 |
D705 D504 |
D706 D504 |
D707 D504 |
D708 D504 |
D709 D504 |
D710 D504 |
D711 D504 |
D712 D504 |
|
E |
D701 D505 |
D702 D505 |
D703 D505 |
D704 D505 |
D705 D505 |
D706 D505 |
D707 D505 |
D708 D505 |
D709 D505 |
D710 D505 |
D711 D505 |
D712 D505 |
|
F |
D701 D506 |
D702 D506 |
D703 D506 |
D704 D506 |
D705 D506 |
D706 D506 |
D707 D506 |
D708 D506 |
D709 D506 |
D710 D506 |
D711 D506 |
D712 D506 |
|
G |
D701 D507 |
D702 D507 |
D703 D507 |
D704 D507 |
D705 D507 |
D706 D507 |
D707 D507 |
D708 D507 |
D709 D507 |
D710 D507 |
D711 D507 |
D712 D507 |
|
H |
D701 D508 |
D702 D508 |
D703 D508 |
D704 D508 |
D705 D508 |
D706 D508 |
D707 D508 |
D708 D508 |
D709 D508 |
D710 D508 |
D711 D508 |
D712 D508 |
The following figures illustrate dual indexing strategies for combining these indexes in 2–16-plex pools.
| • | Circles indicate index adapter combinations that can be combined in a pool. |
| • | Dark gray indicates wells that are not used for pool plexity ≥ 2, but can be used for one-plex pools (single libraries). |
Two-Plex, Dual-Index
Pool diagonally across adjacent columns and rows. For example: A1 and B2 or A2 and B1.
Three-Plex, Dual-Index
Start at an odd-numbered column and rows A, C, E, and G. Use all wells in a 2 × 2 square except the lower-right well, which is depicted in dark gray.
Four-Plex, Dual-Index
Start at odd-numbered columns and rows A, C, E, and G. Use all four wells in a 2 × 2 square.
Five-Plex, Dual-Index
Start at columns 1, 4, 7, and 10 and rows A, C, E, and G. Use all wells in a 2 × 3 rectangle except the lower-right well, which is depicted in dark gray.
Six-Plex, Dual-Index
Start at columns 1, 4, 7, and 10 and rows A, C, E, and G. Use all six wells in a 2 × 3 rectangle.
Seven-Plex, Dual-Index
Start at columns 1, 5, and 9 and rows A, C, E, and G. Use all wells in a 2 × 4 rectangle except the lower-right well, which is depicted in dark gray.
Eight-Plex, Dual-Index (Option 1)
Start at columns 1, 5, or 9 and rows A, C, E, and G. Use all eight wells in a 2 × 4 rectangle.
Eight-Plex, Dual-Index (Option 2)
Start at odd-numbered columns and rows A and E. Use all eight wells in a 4 × 2 rectangle.
Twelve-Plex, Dual-Index
Start at columns 1, 4, 7, and 10 and rows A and E. Use all 12 wells in a 4 × 3 rectangle.
Sixteen-Plex, Dual-Index
Start at columns 1, 5, and 9 and rows A and E. Use all 16 wells in a 4 × 4 square.
