TruSeq CD Indexes (Single Indexing With Dual Index Adapters)

The following table depicts the Index 1 portion of the plate layout for TruSeq DNA CD Indexes and TruSeq RNA CD Indexes (formerly TruSeq HT Indexes).

	1	2	3	4	5	6	7	8	9	10	11	12
A	D701	D702	D703	D704	D705	D706	D707	D708	D709	D710	D711	D712
B	D701	D702	D703	D704	D705	D706	D707	D708	D709	D710	D711	D712
C	D701	D702	D703	D704	D705	D706	D707	D708	D709	D710	D711	D712
D	D701	D702	D703	D704	D705	D706	D707	D708	D709	D710	D711	D712
E	D701	D702	D703	D704	D705	D706	D707	D708	D709	D710	D711	D712
F	D701	D702	D703	D704	D705	D706	D707	D708	D709	D710	D711	D712
G	D701	D702	D703	D704	D705	D706	D707	D708	D709	D710	D711	D712
H	D701	D702	D703	D704	D705	D706	D707	D708	D709	D710	D711	D712

The following figures illustrate pooling strategies for libraries ligated with the Index 1 (i7) and Index 2 (i5) adapters, where only Index 1 is sequenced.

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Circles indicate index adapter combinations that can be combined in a pool.

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Dark gray indicates wells that are not used for pool plexity ≥ 2, but can be used for one-plex pools (single libraries).

These strategies are designed for 2–12-plex pools. For 7–11‑plex pools, combine any of the 2–6-plex pools.