TruSeq Single Indexes

TruSeq Single Indexes are available in Set A and Set B. Each set contains 12 index adapters packaged in tubes.

The following tables show strategies for 2–12-plex pooling when using either set. For 5–11‑plex pools, start with a 4-plex option and add any other index adapters from the same set.

Plexity

Option

Set A Index Adapters Only

Set B Index Adapters Only

2

1

AD006 and AD012

Not recommended

2

AD005 and AD019

Not recommended

3

1

AD002, AD007, and AD019

AD001, AD010, and AD020

2

AD005, AD006, and AD015

AD003, AD009, and AD025

3

Two-plex options plus another index adapter from Set A

AD008, AD011, and AD022

4

1

AD005, AD006, AD012, and AD019

AD001, AD008, AD010, and AD011

2

AD002, AD004, AD007, and AD016

AD003, AD009, AD022, and AD027

3

Three-plex options plus another adapter from Set A

Three-plex options plus another index adapter from Set B

Plexity

Option

Set A Index Adapters Only

Set B Index Adapters Only

2

1

AR006 and AR012

Not recommended

2

AR005 and AR019

Not recommended

3

1

AR002, AR007, and AR019

AR001, AR010, and AR020

2

AR005, AR006, and AR015

AR003, AR009, and AR025

3

Two-plex options plus another index adapter from Set A

AR008, AR011, and AR022

4

1

AR005, AR006, AR012, and AR019

AR001, AR008, AR010, and AR011

2

AR002, AR004, AR007, and AR016

AR003, AR009, AR022, and AR027

3

Three-plex options plus another index adapter from Set A

Three-plex options plus another index adapter from Set B