TruSeq Small RNA Indexes
TruSeq Small RNA Indexes A, B, C, and D each contain 12 unique indexes for single indexing.
The following table shows pooling strategies for two to four libraries generated with index adapters from each set. For 5–11‑plex pools, use the 4-plex options with any adapters.
|
Plexity |
Option |
Set A Only |
Set B Only |
Set C Only* |
Set D Only |
|---|---|---|---|---|---|
|
2 |
1 |
RPI6 and RPI12 |
Not recommended |
Not recommended |
RPI37 and RPI45 |
|
3 |
1 |
RPI1, RPI3, and RPI7 |
RPI16, RPI17, and RPI18 |
Not recommended
|
RPI38, RPI44, and RPI46 |
|
2 |
RPI2, RPI4, and RPI8 |
RPI13, RPI17, and RPI23 |
RPI40, RPI47, and RPI48 |
||
|
3 |
Two-plex option with any other adapter |
Not recommended |
Two-plex option with any other adapter |
||
|
4 |
1 |
RPI2, RPI9, RPI10, and PRI11 |
Three-plex option with any other adapter
|
Not recommended
|
RPI37, RPI39, RPI42, and RPI43 |
|
2 |
RPI4, RPI5, RPI6, and RPI7 |
RPI37, RPI38, RPI45, and RPI46 |
|||
|
3 |
Three-plex option with any other adapter |
Three-plex option with any other adapter |
* Always combine TruSeq Small RNA Indexes C with another TruSeq Small RNA Indexes set.
