Data Output and Storage
Illumina sequencing systems are designed for use with a network. The system is not intended to store run data. Therefore, performing a run in standalone mode requires a network connection to transfer run data to a network location. Do not save run data to a local hard drive. The system hard drive is intended for temporary storage before data is transferred automatically. Data saved on the hard drive that are not used by the current run can compromise performance. Refer to Network Connections for information on the transferring run data to network locations.
When data transfer to external storage is complete, the following files appear in the output folder:
| • | RTAComplete.txt |
| • | CopyComplete.txt |
| • | SequenceComplete.txt |
To confirm successful data transfer, make sure that CopyComplete.txt is present and the Process Management screen displays green checkmarks for all processes.
The following tables provide example storage data. Because actual data retention is subject to local policies, confirm conditions before calculating storage needs.
Run sizes and other details vary depending on multiple factors. The numbers provided are intended to be a guide to the relative range of the data footprint.
NovaSeq X and NovaSeq X Plus Systems
|
Functions |
File Type |
25B Flow Cell (TB) |
10B Flow Cell (TB) |
1.5B Flow Cell (TB) |
|---|---|---|---|---|
|
Real-Time Analysis |
BCL |
11.1 |
4.2 |
0.6 |
|
Interop |
0.03 |
0.01 |
0.01 |
|
|
FASTQ |
GZIP |
14.5 |
5.8 |
0.9 |
|
ORA |
2.9 |
1.2 |
0.2 |
|
|
Map/Align |
BAM |
12.7 |
5.1 |
0.8 |
|
CRAM |
4.9 |
2.0 |
0.3 |
|
|
Variant Calling |
GVCF+VCF |
1.1 |
0.4 |
0.1 |
|
Functions |
File Type |
25B Flow Cell (TB) |
10B Flow Cell (TB) |
1.5B Flow Cell (TB) |
|---|---|---|---|---|
|
Real-Time Analysis |
BCL |
5.2 |
2.1 |
0.3 |
|
Interop |
0.01 |
0.01 |
0.01 |
|
|
FASTQ |
GZIP |
7.2 |
2.9 |
0.4 |
|
ORA |
1.4 |
0.6 |
0.1 |
|
|
Map/Align |
BAM |
6.3 |
2.5 |
0.4 |
|
CRAM |
2.5 |
1.0 |
0.1 |
|
|
Variant Calling |
GVCF+VCF |
0.5 |
0.2 |
0.0 |
|
File Type |
P1 (GB) |
P2 (GB) |
P3 (GB) |
P4 (GB) |
|---|---|---|---|---|
|
BCL |
10–15 |
40–60 |
125–180 |
185–275 |
|
FASTQ |
15–20 |
60–85 |
175–260 |
270–390 |
|
ORA |
5–10 |
25–35 |
75–105 |
105–155 |
|
BAM |
15–20 |
50–75 |
150–225 |
230–335 |
|
CRAM |
4–6 |
15–25 |
50–75 |
75–110 |
|
Read Length (bp) |
BCL Files Output (GB) |
BAM Files Output (GB) |
CRAM Files Output (GB) |
FASTQ Files Output (GB) |
|---|---|---|---|---|
|
2x50 |
20 |
50 |
15 |
75 |
|
2x100 |
40 |
75 |
30 |
150 |
|
2x150 |
55 |
150 |
60 |
300 |
NovaSeq 6000 System
|
File Type |
S1 * (GB) |
S2 (GB) |
S4 (GB) |
|---|---|---|---|
|
CBCL |
470 |
930 |
2800 |
|
Interop folder |
1.2 |
2.3 |
7.0 |
|
FASTQ |
570 |
1125 |
3387 |
|
BAM |
530 |
1050 |
3160 |
|
gVCF/VCF |
14 |
28 |
84 |
iSeq 100 System
|
Output File Type |
Approximate Size (MB) |
|---|---|
|
BAM |
600 |
|
BCL |
850 |
|
FASTQ |
850 |
|
gVCF/VCF |
<10 |
|
InterOp |
2.5 |
