MiSeq Integration v8.0 Validation and Troubleshooting
The following sections explain how to validate the installation of the Illumina MiSeq Integration Package v8.0.
The validation involves the following processes:
| • | Running samples through the Library Prep Validation workflow. The workflow contains a single-step protocol that models the library prep required to produce normalized libraries. At the end of the step, the normalized libraries automatically advance to the MiSeq Sequencing v3.0 workflow. |
| • | Running normalized libraries through the MiSeq Sequencing v3.0 workflow. This process validates the following information: |
| – | Automated generation of a sample sheet for use with the MiSeq Control Software (MCS) and Local Run Manager (LRM). |
| – | Automated tracking of the MiSeq sequencing run and parsing of run statistics into Clarity LIMS, including the following information: |
| – | Run status and metrics of sequencing run |
| – | Sequencing run parameters |
| – | Real-Time Analysis (RTA) v1 run directory location and other run-specific information |
The version of Library Prep Validation workflow depends on whether the workflow is delivered by PreConfigured Workflows Package (PCWP) or Illumina Preset Protocols (IPP).
| • | Library Prep Validation v1.1.2 (PCWP) |
| • | Library Prep Validation v2.0.2 (IPP) |
