NextSeq 1000/2000 Integration v2.3 Validation and Troubleshooting

This section explains how to validate the installation of the Illumina NextSeq 1000/2000 Integration Package v2.3.

The validation process involves the following items:

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Running samples through the Library Prep Validation v2.2.0 workflow.

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The workflow contains a single-step protocol that models the library prep workflow required to produce libraries tagged with index sequences. At the end of the step, these libraries are routed to NextSeq 1000/2000 Sequencing v2.3 workflow.

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Running the libraries through the NextSeq 1000/2000 Sequencing v2.3 workflow. This process validates the following information:

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Successful sequential step advancement of samples in the following steps:

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Automatic validation of run setup information before sample sheet generation or planned run creation.

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Automated generation of a sample sheet. The sample sheet is used to start the sequencing run on NextSeq 1000/2000 Control Software (NCS) via Local run mode.

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Automated creation of a planned run on Illumina Connected Analytics. The NextSeq 1000/2000 Control Software retrieves the planned run to start the run via Hybrid or Cloud run mode.

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Automated tracking of the NextSeq 1000/2000 sequencing run and parsing of run statistics from BaseSpace Sequence Hub into Clarity LIMS using the SIS Integration Service.

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Automated tracking of the secondary analysis using DRAGEN (up to BCL Convert only).

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Parsing of demultiplexing metrics from BSSH into Clarity LIMS using SIS integration service.

Before running the validation steps below, these steps assume that the NextSeq 1000/2000 Sequencing v2.3 Integration Package is installed, and that you have imported the default Clarity LIMS configuration.

For information on how the integration works, refer to NextSeq 1000/2000 Integration v2.3 Configuration and User Interaction.