Sample Sheet Generation
Sample sheet generation occurs on the step before the sequencing run Denature and Dilute (NextSeq 500/550 v1.0) step. Samples are placed on the container to be loaded in the instrument. The default configuration provides the Generate bcl2fastq2 NextSeq SampleSheet automation.
The Generate bcl2fastq2 NextSeq SampleSheet automation uses the Template File Generator (DriverFileGenerator.jar) and a template file to generate a CSV format file for use with bcl2fastq2 v2.20.0 analysis software. The sample sheet content is determined by the fields that display on the Record Details screen of the step (in the Step Data table) and the values entered into the fields. Templates can be customized to create the sample sheet. If additional columns are required by the lab, then they can be inserted.