NextSeq 500/550 Integration v2.3 Validation and Troubleshooting
This guide explains how to validate the installation of the Illumina NextSeq 500/550 Integration Package v2.3.
The validation process involves:
| • | Validating the creation of event files. |
| • | Running samples through the Library Prep Validation v2.3.1 workflow. This workflow is installed by Illumina Preset Protocols (IPP) v2.6 and contains a single-step protocol that models the library prep required to produce normalized libraries. At the end of the step, the normalized libraries are automatically advanced to the NextSeq 500/550 Sequencing v1.1 workflow. |
| • | Running normalized libraries through the NextSeq 500/550 Sequencing v1.1 workflow. This validates the automated generation of a sample sheet for use with bcl2fastq2 v2.20.0 analysis software. It also validates the automated tracking of the NextSeq sequencing run and parsing of run statistics into Clarity LIMS, including: |
| – | Run status and metrics of sequencing run |
| – | Sequencing run parameters |
| – | Real Time Analysis v2 (RTA2) run directory location and other run specific information |
