Library Prep Protocol: Library Prep Validation v2.3.0
This single-step protocol models the library prep required to produce normalized libraries that are ready for the NovaSeq 6000 v3.7 workflow.
Run Library Prep Validation v2.3.0
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1.
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In Lab View, locate the Library Prep Validation v2.3.0 protocol. |
Samples are queued for the Library Prep Validation v2.3.0 step.
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2.
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Add the samples to the Ice Bucket and select View Ice Bucket. |
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3.
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On the Ice Bucket screen, select Begin Work. |
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4.
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On the Placement screen, complete the following actions: |
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a.
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In the Placed Samples area on the right, in the Container field, scan or enter the barcode of the destination container. |
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b.
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Select the samples from the Samples to be Placed area on the left side, and drag them to the container wells on the right side. |
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5.
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On the Add Labels screen, the Labels to Add list is populated with labels from the TruSeq HT Adapters v2 (D7-D5) label group. The group is the only label group configured in the integration and is used by default in this step. |
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a.
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Select labels on the left side, and drag them to the container on the right side to assign them to placed samples. |
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b.
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Select Record Details. |
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6.
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On the Record Details screen, complete the following actions: |
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a.
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In the Sample Details table, enter values for Normalized Molarity (nM). Type values individually or copy and paste to enter multiple values from a spreadsheet. |
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b.
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The Sequencing Instrument column indicates the instrument to be used for the sequencing run. Select NovaSeq 3.0. |
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c.
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Populate the fields in this column using one of the following methods: |
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To populate fields individually for each sample, select from the drop-down list in each row. |
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To populate all fields at one time, copy and paste from a spreadsheet. |
Alternatively, select inside the table and press Ctrl + A to select all rows. At the top of the table, select Sequencing Instrument and then select NovaSeq 3.0 from the adjacent drop-down list. Select Apply.
If working with many samples, copy and paste to insert multiple values from a spreadsheet as follows.
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a.
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Select Copy Samples to copy the sample information from Clarity LIMS to the Clipboard. |
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b.
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Open a blank Excel document and paste the contents of the clipboard into the spreadsheet. |
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c.
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In the spreadsheet, enter the Normalized Molarity and Sequencing Instrument values. Select the entire spreadsheet including the headers (Ctrl + A), and copy (Ctrl + C) the information to the Clipboard. |
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d.
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Within Clarity LIMS, select the Paste Samples button. Paste the contents of the clipboard (Ctrl + V) into the Update Table window and then select Update to paste the contents into the Sample Details table. |
Triggers the Next Steps automation, which sets the value of the next step (for all samples) to Remove from workflow. The Routing Script automation expects this value and requires it to advance samples to the next step.
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8.
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On the Assign Next Steps screen in the Samples table, the Next Step value for all samples is prepopulated with Remove from workflow. |
Do not change this value. If Next Step is not set to Remove from workflow, the routing script does not route samples correctly.
On exit from the step, the Routing Script automation is triggered. This automation assigns samples to the first step of the NovaSeq 6000 v3.7 workflow, Define Run Format (NovaSeq 6000 v3.7). This step is the only one in Protocol 1: Run Format (NovaSeq 6000 v3.7).
