QC and Sample Prep
In BaseSpace Clarity LIMS, protocols for QC and Sample Preparation include modules to increase accuracy and facilitate management of the complex library prep components.
| • | Each step includes a graphical interface to facilitate tube-to-plate and plate-to-plate transfers. |
| • | Each step tracks the user, time, and date of each task being completed in the lab. |
| • | Each step allows printing a sample or plate manifest to use as needed. |
In BaseSpace Clarity LIMS, you can track lot numbers, kit numbers, and other conditions for each laboratory process.
Initial DNA QC
The initial DNA QC protocol contains steps to track quality control data from commonly used QC instruments. Through data integrations, QC data can be added to Clarity LIMS directly from the instrument outputs.
Highlights of this protocol include:
| • | Data integrations for Bioanalyzer 2100, Nanodrop 8000, CaliperGX, and Tapestation 2200, including creation of the input file and parsing of data output. |
| • | Assignment of pass/fail QC based on chosen criteria. |
| • | Aggregation of data from multiple QC steps. |
Protocol Steps
Initial RNA QC
The initial RNA QC protocol contains steps to track quality control data from commonly used QC instruments. Through data integrations, QC data can be added to Clarity LIMS directly from the instrument outputs.
Highlights of this protocol include:
| • | Data integrations for Bioanalyzer 2100, Nanodrop 8000, CaliperGX, and Tapestation 2200, including creation of the input file and parsing of data output. |
| • | Assignment of pass/fail QC based on chosen criteria. |
| • | Aggregation of data from multiple QC steps. |
Protocol Steps
Library Validation QC
The Library Validation QC protocol contains steps to track quality control data from commonly used QC instruments. Through data integrations, QC data can be added to Clarity LIMS directly from the instrument outputs.
Highlights of this protocol include:
| • | Data integrations for Bioanalyzer 2100 and Tapestation 2200, including creation of the input file and parsing of data output. |
| • | Possible integrations with qPCR instruments: TaqMan 7900 or ViiA7. |
| • | Assignment of pass/fail QC based on chosen criteria. |
| • | Aggregation of data from multiple QC steps. |
Protocol Steps
Initial Sample Preparation
The Initial Sample Preparation protocol provides examples of how sample preparation methods can be tracked in BaseSpace Clarity LIMS.
Highlights of this protocol include:
| • | Examples of sample preparation methodologies. |
| • | Steps and protocols that can be modified or used as templates. |
Protocol Steps
Immunohistochemistry
The Immunohistochemistry protocol contains steps to track the major steps needed to stain and image tissue sections.
Highlights of this protocol include:
| • | Track sample placement onto slides. |
| • | Placeholders for image files to be uploaded. |
Protocol steps
Fluorescence in Situ Hybridization
This protocol contains steps to track the major steps of fluorescence in situ hybridization.
Highlights of this protocol include:
| • | Track sample placement onto slides. |
| • | Placeholders for image files to be uploaded. |
Protocol Steps
