FASTQ Files

FASTQ (*.fastq.gz, *.fastq.ora) is a text-based file format containing base calls and quality values per read. Each file contains the following information:

The sample identifier
The sequence
A plus sign (+)
The Phred quality scores in an ASCII + 33 encoded format

The sample identifier is formatted as follows.

@Instrument:RunID:FlowCellID:Lane:Tile:X:Y ReadNum:FilterFlag:0:SampleNumber

Example:

@SIM:1:FCX:1:15:6329:1045 1:N:0:2

TCGCACTCAACGCCCTGCATATGACAAGACAGAATC

+

<>;##=><9=AAAAAAAAAA9#:<#<;<<<????#=