FASTQ Files
FASTQ (*.fastq.gz, *.fastq.ora) is a text-based file format containing base calls and quality values per read. Each file contains the following information:
• | The sample identifier |
• | The sequence |
• | A plus sign (+) |
• | The Phred quality scores in an ASCII + 33 encoded format |
The sample identifier is formatted as follows.
@Instrument:RunID:FlowCellID:Lane:Tile:X:Y ReadNum:FilterFlag:0:SampleNumber
Example:
@SIM:1:FCX:1:15:6329:1045 1:N:0:2
TCGCACTCAACGCCCTGCATATGACAAGACAGAATC
+
<>;##=><9=AAAAAAAAAA9#:<#<;<<<????#=