Start from BCL Files

The run folder contains data from the sequencing run. To run the DRAGEN TruSight Oncology 500 ctDNA Local Analysis Software using BCL files, make sure that the run folder contains the following files:

Folder/File

Description

Config folder

Configuration files.

Data folder

BCL files.

Images folder

Raw sequencing image files.

Interop folder

Interop metric files.

Logs folder

Sequencing system log files.

RTALogs folder

Real-Time Analysis (RTA) log files.

RunInfo.xml file

Run information.

RunParameters.xml file

Run parameters.

SampleSheet.csv file

Sample information. If you want to use a sample sheet that is not in the run folder, provide the full path.

CopyComplete.txt file

Indicates that the sequencing run is finished and data has completed copying.

Use the following command to run the DRAGEN TruSight Oncology 500 ctDNA Local Analysis Software starting from BCL files:

DRAGEN_TruSight_Oncology_500_ctDNA.sh ‑‑runFolder <FULL_PATH_TO_RUN_FOLDER>