Fusions CSV

File name: {Sample_ID}_Fusions.csv

The fusions file contains all candidate fusions identified by the analysis pipeline.

The fusion columns are described in the following table. If you use Microsoft Excel to view this file, genes that are convertible to dates (for example, MARCH1) automatically convert to dd‑mm format (1-Mar).

Fusion Object Field	Description
Sample	Input sample ID.
Name	Fusion name as reported by the DRAGEN fusion caller.
Chr1	The chromosome of the first breakend.
Pos1	The position of the first breakend.
Chr 2	The chromosome of the second breakend.
Pos2	The position of the second breakend.
Direction	The direction of how the breakends are joined together.
Alt_Depth	The number of read-pairs supporting the fusion call.
Total_Depth	Max number of read-pairs aligned to a fusion breakend.
BP1_Depth	Number of read-pairs aligned to the first breakend..
BP2_Depth	Number of read-pairs aligned to the second breakend.
VAF	Variant allele frequency.
Gene1	Genes that overlap the first breakend.
Gene2	Genes that overlap the second breakend.
Contig	The fusion contig.
Filter	Indicates whether the fusion has passed all of the fusion filters.
Is_Cosmic_GenePair	Indicates whether the gene pair has been reported by COSMIC (True/False).
Fusion Directionality Known	Indicates whether the fusion direction is known, and indicated by the order of the genes (True/False).

The following table lists the meaning of the values in the direction column. The values are in the format used by Samtools.

Direction	VCF Format	Description
L1R2	t[p[	The left of breakend1 is joined with the right of breakend2.
L1rL2	t]p]	The left of breakend1 is joined with the reverse complement of the left of breakend2.
L2R1	]p]t	The left of breakend2 is joined with the right of breakend1.
rR2R1	[p[t	The reverse complement of the right of breakend2 is joined with the right of breakend1.