Autolaunch DRAGEN TSO 500 ctDNA Analysis Software Analysis
Autolaunch requires additional BaseSpace Sequence Hub and sample sheet settings.
BaseSpace Sequence Hub Requirements for ICA Autolaunch
Autolaunch uses BaseSpace Sequence Hub to create and export a specially configured sample sheet to enable streaming of sequencing run data to the project and requires the following additional settings.
| • | Access to BaseSpace Sequence Hub. |
| • | ICA Run Storage is enabled under BaseSpace Sequence Hub settings. |
Refer to the BaseSpace Sequence Hub support site page for information on setting up a BaseSpace Sequence Hub project.
Illumina Cloud Run Planning Requirements
Autolaunch requires a sample sheet v2 with specific parameters that instruct the instrument to automatically initiate a DRAGEN TruSight Oncology 500 ctDNA analysis.
Use the run planning option in BaseSpace Sequence Hub to generate the sample sheet. The exported sample sheet is automatically populated with the required fields. Refer to Additional Sample Sheet Fields for Autolaunch for descriptions of the added fields.
For NovaSeq X,
Enter the following required run parameters in BaseSpace Sequence Hub Run Planning:
|
Parameter Name |
Setting |
|---|---|
|
Secondary Analysis |
BaseSpace Sequence Hub / Illumina Connected Analytics |
|
Application |
DRAGEN TSO 500 ctDNA Analysis Software |
To avoid invalid sample sheet configurations, Illumina recommends using the run planning function in BaseSpace Sequence Hub to generate sample sheets. Using an invalid sample sheet can result in failed runs and analyses.
For more information on run planning, refer to the BaseSpace Sequence Hub support site page.
*The BaseSpace Sequence Hub setting for run monitoring and storage must be selected on the instrument to use DRAGEN TSO 500 ctDNA Analysis Software analysis Autolaunch. For information on preparing your instrument for DRAGEN TSO 500 ctDNA Analysis Software Autolaunch, refer to the documentation for your instrument.
| 1. | Use Run Planning in BaseSpace Sequence Hub to create and export a sample sheet. |
| 2. | Import the sample sheet to the instrument and start the sequencing run. |
Data is uploaded to BaseSpace Sequence Hub and then pushed to ICA. You can monitor the run in BaseSpace Sequence Hub.
| 3. | When sequencing and the upload completes, analysis autolaunches in ICA. |
You can monitor the status of the analysis in BaseSpace Sequence Hub or ICA
| 4. | If necessary, requeue the analysis via the run's Summary page in BaseSpace Sequence Hub. |
Refer to the BaseSpace Sequence Hub support site page for more information on requeuing an analysis.
| 5. | View the analysis output results in either BaseSpace Sequence Hub or ICA. |
