Starting From BCL Files

If starting from BCL (*.bcl) files, DRAGEN TruSight Oncology 500 Analysis Software requires the run folder to contain certain files and folders. These inputs are required for Docker.

The run folder contains data from the sequencing run. If starting from BCL files, make sure that the folder contains the following files:

Folder/File

Description

Config folder

Configuration files.

Data folder

*.bcl files.

Images folder

[Optional] Raw sequencing image files.

Interop folder

Interop metric files.

Logs folder

[Optional] Sequencing system log files.

RTALogs folder

Real-Time Analysis (RTA) log files.

RunInfo.xml file

Run information.

RunParameters.xml file

Run parameters.

SampleSheet.csv file

Sample information. If you want to use a sample sheet that is not in the run folder or a sample sheet named something other than SampleSheet.csv, provide the full path.