Metrics Output

The MetricsOutput.tsv file contains the following quality control metrics for all samples:

• DNA library QC metrics for:
– Small variant calling
– TMB
– MSI
– CNV
– GIS (if TruSight Oncology 500 HRD is run)
• RNA library QC metrics
• Run QC metrics, analysis status, and contamination

This TSV file also includes expanded DNA library QC metrics per sample, based on total reads, collapsed reads, chimeric reads, and on-target reads. Analysis using RNA samples also produces RNA library QC metrics and expanded RNA library QC metrics per sample based on total reads and coverage.

The MetricsOutput.tsv file is a final combined metrics report with sample status, key analysis metrics, and metadata in a *.tsv file. Sample metrics within the report include suggested lower specification limits (LSL) and upper specification limits (USL) for each sample in the run.

For troubleshooting information, refer to Troubleshooting.